Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 11949 and 11950 are separated by 293 nucleotides11950 and 11951 are separated by 788 nucleotides11951 and 11952 are separated by 299 nucleotides11952 and 11953 are separated by 731 nucleotides mRNA_3581: 11949 - HMMPfam-Polysaccharide deacetylase-PF01522,ProSiteProfiles-NodB homology domain profile.-PS51677,SUPERFAMILY--SSF88713, at 97,924 to 100,153 11949 mRNA_3582: 11950 - HMMPfam-Polycystin cation channel-PF08016, at 100,447 to 103,297 11950 mRNA_3583: 11951 - Hypothetical Protein, at 104,086 to 104,307 11951 mRNA_3584: 11952 - K00122 FDH formate dehydrogenase, at 104,607 to 106,241 11952 mRNA_3585: 11953 - KOG1339 Aspartyl protease, at 106,973 to 108,703 11953
Group Condition mRNA_3581 mRNA_3582 mRNA_3583 mRNA_3584 mRNA_3585
11949 11950 11951 11952 11953
growth on ypd YPD -0.2 -0.1 N.D. +0.3 -0.6
ypd buoyancy 40 hrs High Buoyancy +0.0 +0.0 N.D. -0.1 -0.4
auxotrophy Dropout Complete +0.1 +0.1 N.D. +0.1 -0.7
growth on oleic acid YNB Oleic Acid +0.1 +0.0 N.D. +0.2 -0.3
ypd buoyancy 40 hrs Low Buoyancy +0.1 -0.0 N.D. -0.1 +0.2
ypd buoyancy 40 hrs No Buoyancy Separation -0.0 +0.1 N.D. -0.1 +0.2
growth on oleic acid YNB Glucose -0.0 +0.2 N.D. +0.3 -0.2
auxotrophy YNB Glucose +0.1 +0.1 N.D. -0.1 +0.2
facs on lipid accumulation Sorted High BODIPY Gate -0.0 -0.1 N.D. +0.6 +0.2
growth on ricinoleic acid YNB Glucose +0.1 +0.4 N.D. +0.1 +0.1
auxotrophy YNB Glucose 75mM Arginine -0.0 +0.2 N.D. +0.1 +0.5
auxotrophy YNB Glucose 75mM Methionine +0.1 +0.2 N.D. +0.3 +0.2
growth on ricinoleic acid YNB Ricinoleic Acid +0.3 +0.3 N.D. +0.3 +0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.2 -0.0 N.D. +0.2 +0.8
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.1 -0.1 N.D. +0.4 +0.8
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.2 -0.1 N.D. -0.1 +1.2
facs on lipid accumulation Sorted No BODIPY Gate -0.1 +0.6 N.D. +0.5 +0.3
growth on ricinoleic acid YNB Methylricinoleic Acid -0.1 +0.3 N.D. +0.1 +1.0
ypd buoyancy 40 hrs Median Buoyancy +0.3 +0.3 N.D. +0.3 +0.5
facs on lipid accumulation Sorted Low BODIPY Gate -0.0 +0.3 N.D. +0.1 +1.3
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.3 +0.2 N.D. +0.7 +1.2
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