Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 11950 and 11951 are separated by 788 nucleotides11951 and 11952 are separated by 299 nucleotides11952 and 11953 are separated by 731 nucleotides11953 and 11954 are separated by 1442 nucleotides mRNA_3582: 11950 - HMMPfam-Polycystin cation channel-PF08016, at 100,447 to 103,297 11950 mRNA_3583: 11951 - Hypothetical Protein, at 104,086 to 104,307 11951 mRNA_3584: 11952 - K00122 FDH formate dehydrogenase, at 104,607 to 106,241 11952 mRNA_3585: 11953 - KOG1339 Aspartyl protease, at 106,973 to 108,703 11953 mRNA_3586: 11954 - HMMPfam-Membrane fusion protein Use1-PF09753, at 110,146 to 111,411 11954
Group Condition mRNA_3582 mRNA_3583 mRNA_3584 mRNA_3585 mRNA_3586
11950 11951 11952 11953 11954
auxotrophy Dropout Complete +0.1 N.D. +0.1 -0.7 N.D.
ypd buoyancy 40 hrs High Buoyancy +0.0 N.D. -0.1 -0.4 N.D.
growth on ypd YPD -0.1 N.D. +0.3 -0.6 N.D.
growth on oleic acid YNB Oleic Acid +0.0 N.D. +0.2 -0.3 N.D.
ypd buoyancy 40 hrs Low Buoyancy -0.0 N.D. -0.1 +0.2 N.D.
auxotrophy YNB Glucose +0.1 N.D. -0.1 +0.2 N.D.
ypd buoyancy 40 hrs No Buoyancy Separation +0.1 N.D. -0.1 +0.2 N.D.
growth on oleic acid YNB Glucose +0.2 N.D. +0.3 -0.2 N.D.
growth on ricinoleic acid YNB Glucose +0.4 N.D. +0.1 +0.1 N.D.
growth on ricinoleic acid YNB Ricinoleic Acid +0.3 N.D. +0.3 +0.1 N.D.
facs on lipid accumulation Sorted High BODIPY Gate -0.1 N.D. +0.6 +0.2 N.D.
auxotrophy YNB Glucose 75mM Methionine +0.2 N.D. +0.3 +0.2 N.D.
auxotrophy YNB Glucose 75mM Arginine +0.2 N.D. +0.1 +0.5 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.0 N.D. +0.2 +0.8 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.1 N.D. -0.1 +1.2 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.1 N.D. +0.4 +0.8 N.D.
ypd buoyancy 40 hrs Median Buoyancy +0.3 N.D. +0.3 +0.5 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid +0.3 N.D. +0.1 +1.0 N.D.
facs on lipid accumulation Sorted No BODIPY Gate +0.6 N.D. +0.5 +0.3 N.D.
facs on lipid accumulation Sorted Low BODIPY Gate +0.3 N.D. +0.1 +1.3 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.2 N.D. +0.7 +1.2 N.D.
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