Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 11953 and 11954 are separated by 1442 nucleotides11954 and 11955 are separated by 181 nucleotides11955 and 11956 are separated by 244 nucleotides11956 and 11957 are separated by 485 nucleotides mRNA_3585: 11953 - KOG1339 Aspartyl protease, at 106,973 to 108,703 11953 mRNA_3586: 11954 - HMMPfam-Membrane fusion protein Use1-PF09753, at 110,146 to 111,411 11954 mRNA_3587: 11955 - K12836 U2AF1 splicing factor U2AF 35 kDa subunit, at 111,593 to 112,967 11955 mRNA_3588: 11956 - KOG0254 Predicted transporter (major facilitator superfamily), at 113,212 to 115,744 11956 mRNA_3589: 11957 - HMMPfam-F-box domain-PF00646,ProSiteProfiles-F-box domain profile.-PS50181,SMART-A Receptor for Ubiquitination Targets-SM00256,SUPERFAMILY--SSF101908,SUPERFAMILY--SSF81383, at 116,230 to 117,801 11957
Group Condition mRNA_3585 mRNA_3586 mRNA_3587 mRNA_3588 mRNA_3589
11953 11954 11955 11956 11957
ypd buoyancy 40 hrs High Buoyancy -0.4 N.D. N.D. -0.2 +0.1
growth on ypd YPD -0.6 N.D. N.D. +0.3 -0.2
ypd buoyancy 40 hrs No Buoyancy Separation +0.2 N.D. N.D. -0.2 -0.2
growth on ricinoleic acid YNB Ricinoleic Acid +0.1 N.D. N.D. +0.1 -0.2
growth on ricinoleic acid YNB Glucose +0.1 N.D. N.D. +0.3 -0.2
growth on oleic acid YNB Glucose -0.2 N.D. N.D. +0.2 +0.3
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.8 N.D. N.D. +0.1 -0.6
facs on lipid accumulation Sorted No BODIPY Gate +0.3 N.D. N.D. +0.0 +0.0
auxotrophy Dropout Complete -0.7 N.D. N.D. +0.2 +0.9
auxotrophy YNB Glucose +0.2 N.D. N.D. +0.1 +0.2
ypd buoyancy 40 hrs Low Buoyancy +0.2 N.D. N.D. -0.1 +0.3
growth on oleic acid YNB Oleic Acid -0.3 N.D. N.D. +0.2 +0.5
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +1.2 N.D. N.D. +0.1 -0.9
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.8 N.D. N.D. +0.3 -0.5
ypd buoyancy 40 hrs Median Buoyancy +0.5 N.D. N.D. +0.0 +0.1
auxotrophy YNB Glucose 75mM Arginine +0.5 N.D. N.D. +0.1 +0.2
facs on lipid accumulation Sorted High BODIPY Gate +0.2 N.D. N.D. +0.3 +0.3
buoyancy on lipid accumulation 40 hrs High Buoyancy +1.2 N.D. N.D. +0.1 -0.5
auxotrophy YNB Glucose 75mM Methionine +0.2 N.D. N.D. +0.1 +0.6
growth on ricinoleic acid YNB Methylricinoleic Acid +1.0 N.D. N.D. +0.4 +0.1
facs on lipid accumulation Sorted Low BODIPY Gate +1.3 N.D. N.D. +0.6 -0.4
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