Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 11955 and 11956 are separated by 244 nucleotides11956 and 11957 are separated by 485 nucleotides11957 and 11958 are separated by 1033 nucleotides11958 and 11959 are separated by 272 nucleotides mRNA_3587: 11955 - K12836 U2AF1 splicing factor U2AF 35 kDa subunit, at 111,593 to 112,967 11955 mRNA_3588: 11956 - KOG0254 Predicted transporter (major facilitator superfamily), at 113,212 to 115,744 11956 mRNA_3589: 11957 - HMMPfam-F-box domain-PF00646,ProSiteProfiles-F-box domain profile.-PS50181,SMART-A Receptor for Ubiquitination Targets-SM00256,SUPERFAMILY--SSF101908,SUPERFAMILY--SSF81383, at 116,230 to 117,801 11957 mRNA_3590: 11958 - K02136 ATPeF1G, ATP5C1, ATP3 F-type H+-transporting ATPase subunit gamma, at 118,835 to 120,315 11958 mRNA_3591: 11959 - KOG2614 Kynurenine 3-monooxygenase and related flavoprotein monooxygenases, at 120,588 to 122,766 11959
Group Condition mRNA_3587 mRNA_3588 mRNA_3589 mRNA_3590 mRNA_3591
11955 11956 11957 11958 11959
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. +0.1 -0.9 N.D. -0.1
ypd buoyancy 40 hrs No Buoyancy Separation N.D. -0.2 -0.2 N.D. -0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. +0.1 -0.5 N.D. -0.3
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. +0.1 -0.6 N.D. +0.0
growth on ricinoleic acid YNB Glucose N.D. +0.3 -0.2 N.D. -0.5
growth on ricinoleic acid YNB Ricinoleic Acid N.D. +0.1 -0.2 N.D. -0.4
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. +0.3 -0.5 N.D. -0.1
auxotrophy YNB Glucose 75mM Arginine N.D. +0.1 +0.2 N.D. -0.6
ypd buoyancy 40 hrs High Buoyancy N.D. -0.2 +0.1 N.D. -0.1
auxotrophy YNB Glucose N.D. +0.1 +0.2 N.D. -0.4
ypd buoyancy 40 hrs Median Buoyancy N.D. +0.0 +0.1 N.D. -0.2
growth on ypd YPD N.D. +0.3 -0.2 N.D. -0.3
ypd buoyancy 40 hrs Low Buoyancy N.D. -0.1 +0.3 N.D. -0.1
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. +0.4 +0.1 N.D. -0.4
growth on oleic acid YNB Glucose N.D. +0.2 +0.3 N.D. -0.3
auxotrophy YNB Glucose 75mM Methionine N.D. +0.1 +0.6 N.D. -0.4
growth on oleic acid YNB Oleic Acid N.D. +0.2 +0.5 N.D. -0.3
facs on lipid accumulation Sorted No BODIPY Gate N.D. +0.0 +0.0 N.D. +0.5
facs on lipid accumulation Sorted Low BODIPY Gate N.D. +0.6 -0.4 N.D. +0.6
auxotrophy Dropout Complete N.D. +0.2 +0.9 N.D. -0.1
facs on lipid accumulation Sorted High BODIPY Gate N.D. +0.3 +0.3 N.D. +0.4
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