Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 11964 and 11965 are separated by 179 nucleotides11965 and 11966 are separated by 2287 nucleotides11966 and 11967 are separated by 217 nucleotides11967 and 11968 are separated by 612 nucleotides mRNA_3596: 11964 - HMMPfam-Phytanoyl-CoA dioxygenase (PhyH)-PF05721,SUPERFAMILY--SSF51197, at 131,256 to 132,687 11964 mRNA_3597: 11965 - K15562 BUR1, SGV1 serine/threonine-protein kinase BUR1, at 132,867 to 134,971 11965 mRNA_3598: 11966 - KOG2652 RNA polymerase II transcription initiation factor TFIIA, large chain, at 137,259 to 138,295 11966 mRNA_3599: 11967 - K02134 ATPeF1D, ATP5D, ATP16 F-type H+-transporting ATPase subunit delta, at 138,513 to 139,861 11967 mRNA_3600: 11968 - HMMPfam-PWWP domain-PF00855,ProSiteProfiles-PWWP domain profile.-PS50812,SMART-domain with conserved PWWP motif-SM00293,SUPERFAMILY--SSF63748, at 140,474 to 142,309 11968
Group Condition mRNA_3596 mRNA_3597 mRNA_3598 mRNA_3599 mRNA_3600
11964 11965 11966 11967 11968
facs on lipid accumulation Sorted Low BODIPY Gate +0.4 -0.4 N.D. N.D. -2.0
ypd buoyancy 40 hrs Low Buoyancy -0.2 -0.5 N.D. N.D. -0.6
growth on ricinoleic acid YNB Ricinoleic Acid +0.3 +1.2 N.D. N.D. -2.7
ypd buoyancy 40 hrs Median Buoyancy -0.1 -1.0 N.D. N.D. +0.1
growth on ricinoleic acid YNB Glucose +0.4 +0.4 N.D. N.D. -1.5
ypd buoyancy 40 hrs No Buoyancy Separation -0.4 -0.0 N.D. N.D. -0.3
growth on ricinoleic acid YNB Methylricinoleic Acid +0.3 +0.7 N.D. N.D. -1.7
auxotrophy YNB Glucose -0.2 +0.6 N.D. N.D. -0.9
auxotrophy YNB Glucose 75mM Methionine -0.0 +0.7 N.D. N.D. -1.1
facs on lipid accumulation Sorted No BODIPY Gate +0.1 -0.6 N.D. N.D. +0.3
auxotrophy YNB Glucose 75mM Arginine +0.1 +0.7 N.D. N.D. -1.0
growth on oleic acid YNB Glucose +0.3 +0.5 N.D. N.D. -0.6
ypd buoyancy 40 hrs High Buoyancy -0.1 -0.0 N.D. N.D. +0.3
facs on lipid accumulation Sorted High BODIPY Gate -0.0 +0.9 N.D. N.D. -0.5
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.3 +0.4 N.D. N.D. -0.3
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.4 +0.4 N.D. N.D. -0.4
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.1 +0.5 N.D. N.D. -0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.5 +0.5 N.D. N.D. -0.3
growth on ypd YPD +0.3 -1.3 N.D. N.D. +1.7
auxotrophy Dropout Complete +0.0 +1.2 N.D. N.D. -0.2
growth on oleic acid YNB Oleic Acid +0.3 +1.2 N.D. N.D. -0.3
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