Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 11965 and 11966 are separated by 2287 nucleotides11966 and 11967 are separated by 217 nucleotides11967 and 11968 are separated by 612 nucleotides11968 and 11969 are separated by 927 nucleotides mRNA_3597: 11965 - K15562 BUR1, SGV1 serine/threonine-protein kinase BUR1, at 132,867 to 134,971 11965 mRNA_3598: 11966 - KOG2652 RNA polymerase II transcription initiation factor TFIIA, large chain, at 137,259 to 138,295 11966 mRNA_3599: 11967 - K02134 ATPeF1D, ATP5D, ATP16 F-type H+-transporting ATPase subunit delta, at 138,513 to 139,861 11967 mRNA_3600: 11968 - HMMPfam-PWWP domain-PF00855,ProSiteProfiles-PWWP domain profile.-PS50812,SMART-domain with conserved PWWP motif-SM00293,SUPERFAMILY--SSF63748, at 140,474 to 142,309 11968 mRNA_3601: 11969 - K11367 CHD1 chromodomain-helicase-DNA-binding protein 1, at 143,237 to 148,837 11969
Group Condition mRNA_3597 mRNA_3598 mRNA_3599 mRNA_3600 mRNA_3601
11965 11966 11967 11968 11969
facs on lipid accumulation Sorted Low BODIPY Gate -0.4 N.D. N.D. -2.0 -1.8
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.4 N.D. N.D. -0.4 -3.3
growth on ypd YPD -1.3 N.D. N.D. +1.7 -2.9
growth on oleic acid YNB Glucose +0.5 N.D. N.D. -0.6 -2.3
growth on ricinoleic acid YNB Ricinoleic Acid +1.2 N.D. N.D. -2.7 -0.9
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.5 N.D. N.D. -0.3 -2.5
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.5 N.D. N.D. -0.2 -2.6
growth on ricinoleic acid YNB Glucose +0.4 N.D. N.D. -1.5 -1.0
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.4 N.D. N.D. -0.3 -2.0
facs on lipid accumulation Sorted High BODIPY Gate +0.9 N.D. N.D. -0.5 -1.8
growth on ricinoleic acid YNB Methylricinoleic Acid +0.7 N.D. N.D. -1.7 -0.0
ypd buoyancy 40 hrs Low Buoyancy -0.5 N.D. N.D. -0.6 +0.2
auxotrophy YNB Glucose +0.6 N.D. N.D. -0.9 -0.6
ypd buoyancy 40 hrs Median Buoyancy -1.0 N.D. N.D. +0.1 +0.2
ypd buoyancy 40 hrs High Buoyancy -0.0 N.D. N.D. +0.3 -0.7
facs on lipid accumulation Sorted No BODIPY Gate -0.6 N.D. N.D. +0.3 +0.3
auxotrophy YNB Glucose 75mM Methionine +0.7 N.D. N.D. -1.1 +0.5
growth on oleic acid YNB Oleic Acid +1.2 N.D. N.D. -0.3 -0.9
auxotrophy YNB Glucose 75mM Arginine +0.7 N.D. N.D. -1.0 +0.6
ypd buoyancy 40 hrs No Buoyancy Separation -0.0 N.D. N.D. -0.3 +0.7
auxotrophy Dropout Complete +1.2 N.D. N.D. -0.2 -0.0
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