Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 11966 and 11967 are separated by 217 nucleotides11967 and 11968 are separated by 612 nucleotides11968 and 11969 are separated by 927 nucleotides11969 and 11970 are separated by 505 nucleotides mRNA_3598: 11966 - KOG2652 RNA polymerase II transcription initiation factor TFIIA, large chain, at 137,259 to 138,295 11966 mRNA_3599: 11967 - K02134 ATPeF1D, ATP5D, ATP16 F-type H+-transporting ATPase subunit delta, at 138,513 to 139,861 11967 mRNA_3600: 11968 - HMMPfam-PWWP domain-PF00855,ProSiteProfiles-PWWP domain profile.-PS50812,SMART-domain with conserved PWWP motif-SM00293,SUPERFAMILY--SSF63748, at 140,474 to 142,309 11968 mRNA_3601: 11969 - K11367 CHD1 chromodomain-helicase-DNA-binding protein 1, at 143,237 to 148,837 11969 mRNA_3602: 11970 - K10839 RAD23, HR23 UV excision repair protein RAD23, at 149,343 to 150,921 11970
Group Condition mRNA_3598 mRNA_3599 mRNA_3600 mRNA_3601 mRNA_3602
11966 11967 11968 11969 11970
facs on lipid accumulation Sorted Low BODIPY Gate N.D. N.D. -2.0 -1.8 -0.7
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. N.D. -0.2 -2.6 -0.8
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. N.D. -0.4 -3.3 +0.2
growth on ricinoleic acid YNB Ricinoleic Acid N.D. N.D. -2.7 -0.9 +0.2
growth on oleic acid YNB Glucose N.D. N.D. -0.6 -2.3 +0.1
growth on ricinoleic acid YNB Glucose N.D. N.D. -1.5 -1.0 -0.3
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. N.D. -0.3 -2.5 +0.1
facs on lipid accumulation Sorted High BODIPY Gate N.D. N.D. -0.5 -1.8 -0.4
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. N.D. -1.7 -0.0 -0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. N.D. -0.3 -2.0 +0.5
growth on ypd YPD N.D. N.D. +1.7 -2.9 -0.3
auxotrophy YNB Glucose N.D. N.D. -0.9 -0.6 -0.0
growth on oleic acid YNB Oleic Acid N.D. N.D. -0.3 -0.9 +0.3
ypd buoyancy 40 hrs High Buoyancy N.D. N.D. +0.3 -0.7 -0.4
ypd buoyancy 40 hrs Low Buoyancy N.D. N.D. -0.6 +0.2 -0.1
auxotrophy YNB Glucose 75mM Methionine N.D. N.D. -1.1 +0.5 +0.2
auxotrophy YNB Glucose 75mM Arginine N.D. N.D. -1.0 +0.6 -0.0
auxotrophy Dropout Complete N.D. N.D. -0.2 -0.0 +0.2
ypd buoyancy 40 hrs No Buoyancy Separation N.D. N.D. -0.3 +0.7 -0.3
facs on lipid accumulation Sorted No BODIPY Gate N.D. N.D. +0.3 +0.3 -0.3
ypd buoyancy 40 hrs Median Buoyancy N.D. N.D. +0.1 +0.2 +0.3
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