Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 11970 and 11971 are separated by 208 nucleotides11971 and 11972 are separated by 191 nucleotides11972 and 11973 are separated by 411 nucleotides11973 and 11974 are separated by 138 nucleotides mRNA_3602: 11970 - K10839 RAD23, HR23 UV excision repair protein RAD23, at 149,343 to 150,921 11970 mRNA_3603: 11971 - K13628 iscA, ISCA1 iron-sulfur cluster assembly protein, at 151,130 to 151,909 11971 mRNA_3604: 11972 - K10950 ERO1L ERO1-like protein alpha, at 152,101 to 154,380 11972 mRNA_3605: 11973 - K01872 AARS, alaS alanyl-tRNA synthetase, at 154,792 to 158,003 11973 mRNA_3606: 11974 - HMMPfam-Tho complex subunit 7-PF05615, at 158,142 to 159,312 11974
Group Condition mRNA_3602 mRNA_3603 mRNA_3604 mRNA_3605 mRNA_3606
11970 11971 11972 11973 11974
facs on lipid accumulation Sorted Low BODIPY Gate -0.7 N.D. N.D. N.D. -2.6
facs on lipid accumulation Sorted High BODIPY Gate -0.4 N.D. N.D. N.D. -2.4
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.8 N.D. N.D. N.D. -1.4
facs on lipid accumulation Sorted No BODIPY Gate -0.3 N.D. N.D. N.D. -1.8
growth on ricinoleic acid YNB Ricinoleic Acid +0.2 N.D. N.D. N.D. -1.5
ypd buoyancy 40 hrs Median Buoyancy +0.3 N.D. N.D. N.D. -1.0
ypd buoyancy 40 hrs High Buoyancy -0.4 N.D. N.D. N.D. -0.4
growth on oleic acid YNB Oleic Acid +0.3 N.D. N.D. N.D. -1.0
ypd buoyancy 40 hrs No Buoyancy Separation -0.3 N.D. N.D. N.D. -0.0
auxotrophy YNB Glucose 75mM Arginine -0.0 N.D. N.D. N.D. -0.3
growth on oleic acid YNB Glucose +0.1 N.D. N.D. N.D. -0.4
growth on ricinoleic acid YNB Methylricinoleic Acid -0.2 N.D. N.D. N.D. -0.0
ypd buoyancy 40 hrs Low Buoyancy -0.1 N.D. N.D. N.D. +0.1
growth on ypd YPD -0.3 N.D. N.D. N.D. +0.3
growth on ricinoleic acid YNB Glucose -0.3 N.D. N.D. N.D. +0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.2 N.D. N.D. N.D. -0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.1 N.D. N.D. N.D. +0.3
auxotrophy Dropout Complete +0.2 N.D. N.D. N.D. +0.2
auxotrophy YNB Glucose -0.0 N.D. N.D. N.D. +0.5
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.5 N.D. N.D. N.D. +0.1
auxotrophy YNB Glucose 75mM Methionine +0.2 N.D. N.D. N.D. +0.5
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