Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 11971 and 11972 are separated by 191 nucleotides11972 and 11973 are separated by 411 nucleotides11973 and 11974 are separated by 138 nucleotides11974 and 11975 are separated by 115 nucleotides mRNA_3603: 11971 - K13628 iscA, ISCA1 iron-sulfur cluster assembly protein, at 151,130 to 151,909 11971 mRNA_3604: 11972 - K10950 ERO1L ERO1-like protein alpha, at 152,101 to 154,380 11972 mRNA_3605: 11973 - K01872 AARS, alaS alanyl-tRNA synthetase, at 154,792 to 158,003 11973 mRNA_3606: 11974 - HMMPfam-Tho complex subunit 7-PF05615, at 158,142 to 159,312 11974 mRNA_3607: 11975 - BLAST putative transmembrane protein [Rhizoctonia solani 123E], at 159,428 to 160,353 11975
Group Condition mRNA_3603 mRNA_3604 mRNA_3605 mRNA_3606 mRNA_3607
11971 11972 11973 11974 11975
facs on lipid accumulation Sorted Low BODIPY Gate N.D. N.D. N.D. -2.6 -1.8
facs on lipid accumulation Sorted No BODIPY Gate N.D. N.D. N.D. -1.8 -0.4
facs on lipid accumulation Sorted High BODIPY Gate N.D. N.D. N.D. -2.4 +0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. N.D. N.D. -1.4 -0.4
growth on ricinoleic acid YNB Ricinoleic Acid N.D. N.D. N.D. -1.5 -0.2
ypd buoyancy 40 hrs Median Buoyancy N.D. N.D. N.D. -1.0 -0.1
growth on oleic acid YNB Oleic Acid N.D. N.D. N.D. -1.0 -0.0
ypd buoyancy 40 hrs No Buoyancy Separation N.D. N.D. N.D. -0.0 -0.8
auxotrophy YNB Glucose 75mM Arginine N.D. N.D. N.D. -0.3 -0.4
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. N.D. N.D. +0.1 -0.9
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. N.D. N.D. -0.1 -0.7
ypd buoyancy 40 hrs High Buoyancy N.D. N.D. N.D. -0.4 -0.4
growth on oleic acid YNB Glucose N.D. N.D. N.D. -0.4 +0.0
growth on ricinoleic acid YNB Glucose N.D. N.D. N.D. +0.2 -0.3
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. N.D. N.D. -0.0 -0.0
auxotrophy Dropout Complete N.D. N.D. N.D. +0.2 -0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. N.D. N.D. +0.3 -0.2
growth on ypd YPD N.D. N.D. N.D. +0.3 -0.1
auxotrophy YNB Glucose 75mM Methionine N.D. N.D. N.D. +0.5 -0.2
auxotrophy YNB Glucose N.D. N.D. N.D. +0.5 -0.2
ypd buoyancy 40 hrs Low Buoyancy N.D. N.D. N.D. +0.1 +0.2
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