Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 12016 and 12017 are separated by 599 nucleotides12017 and 12018 are separated by 946 nucleotides12018 and 12019 are separated by 222 nucleotides12019 and 12020 are separated by 632 nucleotides mRNA_3648: 12016 - K15448 TRM112, TRMT112 multifunctional methyltransferase subunit TRM112, at 252,546 to 253,342 12016 mRNA_3649: 12017 - HMMPfam-Protein of unknown function, DUF604-PF04646, at 253,942 to 255,854 12017 mRNA_3650: 12018 - Hypothetical Protein, at 256,801 to 258,115 12018 mRNA_3651: 12019 - BLAST putative protein [Melanopsichium pennsylvanicum 4], at 258,338 to 258,829 12019 mRNA_3652: 12020 - K17278 PGRMC1_2 membrane-associated progesterone receptor component, at 259,462 to 260,271 12020
Group Condition mRNA_3648 mRNA_3649 mRNA_3650 mRNA_3651 mRNA_3652
12016 12017 12018 12019 12020
auxotrophy YNB Glucose 75mM Arginine N.D. +0.3 -0.3 -1.0 -0.1
facs on lipid accumulation Sorted High BODIPY Gate N.D. -0.6 +0.3 -0.4 +0.0
growth on oleic acid YNB Oleic Acid N.D. -0.0 -0.1 -0.2 -0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. +0.1 -0.3 -0.1 -0.1
growth on oleic acid YNB Glucose N.D. +0.4 -0.1 -0.3 -0.3
auxotrophy YNB Glucose 75mM Methionine N.D. +0.3 -0.2 -0.0 -0.2
auxotrophy YNB Glucose N.D. +0.6 -0.5 -0.3 +0.1
auxotrophy Dropout Complete N.D. +0.5 -0.3 -0.2 -0.1
growth on ypd YPD N.D. +0.4 -0.0 -0.2 -0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. +0.0 -0.1 +0.4 -0.1
facs on lipid accumulation Sorted No BODIPY Gate N.D. +0.1 +0.3 +0.1 -0.4
facs on lipid accumulation Sorted Low BODIPY Gate N.D. -0.0 +0.3 +0.5 -0.6
ypd buoyancy 40 hrs High Buoyancy N.D. +0.0 +0.1 -0.2 +0.3
growth on ricinoleic acid YNB Ricinoleic Acid N.D. +0.8 -0.4 -0.5 +0.3
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. +0.1 -0.1 +0.4 -0.2
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. +1.0 -0.3 -0.4 +0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. +0.0 -0.3 +0.4 +0.3
growth on ricinoleic acid YNB Glucose N.D. +1.0 -0.3 -0.1 -0.2
ypd buoyancy 40 hrs Low Buoyancy N.D. +0.8 -0.0 -0.3 +0.2
ypd buoyancy 40 hrs Median Buoyancy N.D. +0.5 +0.1 +0.1 +0.2
ypd buoyancy 40 hrs No Buoyancy Separation N.D. +1.4 +0.4 -0.0 -0.5
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