Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 12031 and 12032 are separated by 901 nucleotides12032 and 12033 are separated by 105 nucleotides12033 and 12034 are separated by 1647 nucleotides12034 and 12035 are separated by 233 nucleotides mRNA_3663: 12031 - K01738 cysK cysteine synthase A, at 288,181 to 289,942 12031 mRNA_3664: 12032 - K15111 SLC25A26 solute carrier family 25 (mitochondrial S-adenosylmethionine transporter), member 26, at 290,844 to 292,094 12032 mRNA_3665: 12033 - HMMPfam-CorA-like Mg2+ transporter protein-PF01544,SUPERFAMILY--SSF143865,SUPERFAMILY--SSF144083, at 292,200 to 295,663 12033 mRNA_3666: 12034 - K16055 TPS trehalose 6-phosphate synthase/phosphatase, at 297,311 to 299,921 12034 mRNA_3667: 12035 - KOG3455 Predicted membrane protein, at 300,155 to 301,036 12035
Group Condition mRNA_3663 mRNA_3664 mRNA_3665 mRNA_3666 mRNA_3667
12031 12032 12033 12034 12035
facs on lipid accumulation Sorted High BODIPY Gate -0.2 -4.4 +0.2 -3.3 N.D.
facs on lipid accumulation Sorted No BODIPY Gate -0.9 -4.5 +0.3 -1.1 N.D.
growth on oleic acid YNB Glucose -2.4 -4.2 -0.2 +0.6 N.D.
facs on lipid accumulation Sorted Low BODIPY Gate -2.3 -4.1 -0.2 +0.5 N.D.
growth on ricinoleic acid YNB Glucose -2.8 -3.3 -0.1 +0.2 N.D.
auxotrophy YNB Glucose 75mM Arginine -3.1 -2.5 -0.3 +0.1 N.D.
auxotrophy YNB Glucose -2.9 -2.8 -0.2 +0.5 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy -1.3 -2.1 -0.4 -0.3 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid -3.3 -0.6 -0.2 +0.0 N.D.
growth on ricinoleic acid YNB Ricinoleic Acid -2.6 -2.2 +0.1 +1.0 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.9 -2.3 -0.1 -0.1 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy -2.3 +0.1 +0.0 -0.4 N.D.
auxotrophy YNB Glucose 75mM Methionine -0.5 -1.7 -0.1 +0.4 N.D.
ypd buoyancy 40 hrs No Buoyancy Separation +0.3 -2.2 +0.6 -0.2 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy +1.0 -1.7 -0.0 -0.4 N.D.
growth on ypd YPD -0.5 -0.1 +0.0 -0.3 N.D.
ypd buoyancy 40 hrs Low Buoyancy +0.4 -0.5 +0.1 -0.1 N.D.
ypd buoyancy 40 hrs High Buoyancy +0.5 -0.2 -0.1 +0.3 N.D.
growth on oleic acid YNB Oleic Acid -0.5 +0.9 -0.2 +0.6 N.D.
auxotrophy Dropout Complete -0.6 +1.7 -0.3 +0.2 N.D.
ypd buoyancy 40 hrs Median Buoyancy +0.5 +0.5 +0.1 -0.1 N.D.
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