Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 12033 and 12034 are separated by 1647 nucleotides12034 and 12035 are separated by 233 nucleotides12035 and 12036 are separated by 121 nucleotides12036 and 12037 are separated by 164 nucleotides mRNA_3665: 12033 - HMMPfam-CorA-like Mg2+ transporter protein-PF01544,SUPERFAMILY--SSF143865,SUPERFAMILY--SSF144083, at 292,200 to 295,663 12033 mRNA_3666: 12034 - K16055 TPS trehalose 6-phosphate synthase/phosphatase, at 297,311 to 299,921 12034 mRNA_3667: 12035 - KOG3455 Predicted membrane protein, at 300,155 to 301,036 12035 mRNA_3668: 12036 - K08870 TWF twinfilin, at 301,158 to 305,676 12036 mRNA_3669: 12037 - KOG1756 Histone 2A, at 305,841 to 306,858 12037
Group Condition mRNA_3665 mRNA_3666 mRNA_3667 mRNA_3668 mRNA_3669
12033 12034 12035 12036 12037
facs on lipid accumulation Sorted High BODIPY Gate +0.2 -3.3 N.D. +0.0 +0.9
growth on ypd YPD +0.0 -0.3 N.D. -0.1 -0.3
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.4 -0.3 N.D. +0.1 -0.0
auxotrophy YNB Glucose 75mM Arginine -0.3 +0.1 N.D. +0.0 -0.3
ypd buoyancy 40 hrs High Buoyancy -0.1 +0.3 N.D. +0.0 -0.6
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.0 -0.4 N.D. -0.1 +0.2
growth on ricinoleic acid YNB Methylricinoleic Acid -0.2 +0.0 N.D. +0.0 -0.1
facs on lipid accumulation Sorted No BODIPY Gate +0.3 -1.1 N.D. -0.0 +0.6
ypd buoyancy 40 hrs Median Buoyancy +0.1 -0.1 N.D. -0.1 -0.0
ypd buoyancy 40 hrs Low Buoyancy +0.1 -0.1 N.D. -0.1 +0.1
auxotrophy Dropout Complete -0.3 +0.2 N.D. +0.1 +0.0
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.0 -0.4 N.D. +0.1 +0.5
auxotrophy YNB Glucose 75mM Methionine -0.1 +0.4 N.D. +0.1 -0.1
auxotrophy YNB Glucose -0.2 +0.5 N.D. +0.1 +0.0
growth on oleic acid YNB Glucose -0.2 +0.6 N.D. +0.1 -0.0
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.1 -0.1 N.D. -0.1 +0.9
growth on ricinoleic acid YNB Glucose -0.1 +0.2 N.D. +0.2 +0.4
ypd buoyancy 40 hrs No Buoyancy Separation +0.6 -0.2 N.D. +0.4 +0.3
growth on oleic acid YNB Oleic Acid -0.2 +0.6 N.D. +0.2 +0.4
facs on lipid accumulation Sorted Low BODIPY Gate -0.2 +0.5 N.D. +0.1 +0.6
growth on ricinoleic acid YNB Ricinoleic Acid +0.1 +1.0 N.D. +0.3 +0.1
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