Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 12042 and 12043 are separated by 477 nucleotides12043 and 12044 are separated by 1433 nucleotides12044 and 12045 are separated by 482 nucleotides12045 and 12046 are separated by 719 nucleotides mRNA_3674: 12042 - K00128 ALDH aldehyde dehydrogenase (NAD+), at 314,540 to 316,836 12042 mRNA_3675: 12043 - K11251 H2A histone H2A, at 317,314 to 317,987 12043 mRNA_3676: 12044 - K11833 USP2_21 ubiquitin carboxyl-terminal hydrolase 2/21, at 319,421 to 320,803 12044 mRNA_3677: 12045 - KOG0266 WD40 repeat-containing protein, at 321,286 to 323,880 12045 mRNA_3678: 12046 - K14530 RPP40 ribonucleases P/MRP protein subunit RPP40, at 324,600 to 326,289 12046
Group Condition mRNA_3674 mRNA_3675 mRNA_3676 mRNA_3677 mRNA_3678
12042 12043 12044 12045 12046
growth on ricinoleic acid YNB Ricinoleic Acid -0.3 -0.3 N.D. -0.4 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid +0.2 -0.3 N.D. -0.7 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.4 -0.0 N.D. -0.1 N.D.
ypd buoyancy 40 hrs Low Buoyancy -0.2 +0.0 N.D. -0.3 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.3 +0.3 N.D. -0.3 N.D.
growth on oleic acid YNB Oleic Acid +0.1 -0.1 N.D. -0.4 N.D.
auxotrophy YNB Glucose 75mM Methionine +0.5 -0.2 N.D. -0.5 N.D.
growth on oleic acid YNB Glucose +0.1 -0.2 N.D. -0.1 N.D.
growth on ricinoleic acid YNB Glucose +0.3 -0.1 N.D. -0.5 N.D.
auxotrophy YNB Glucose 75mM Arginine +0.5 -0.3 N.D. -0.4 N.D.
facs on lipid accumulation Sorted High BODIPY Gate -0.0 +0.2 N.D. -0.4 N.D.
ypd buoyancy 40 hrs No Buoyancy Separation -1.1 +0.4 N.D. +0.5 N.D.
growth on ypd YPD -0.2 +0.1 N.D. +0.0 N.D.
auxotrophy Dropout Complete +0.5 -0.4 N.D. -0.1 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.1 +0.3 N.D. -0.1 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.1 +0.2 N.D. -0.1 N.D.
ypd buoyancy 40 hrs Median Buoyancy -0.0 +0.1 N.D. +0.2 N.D.
auxotrophy YNB Glucose +0.4 +0.4 N.D. -0.4 N.D.
facs on lipid accumulation Sorted No BODIPY Gate -0.1 +0.0 N.D. +0.6 N.D.
ypd buoyancy 40 hrs High Buoyancy -0.1 +0.7 N.D. +0.3 N.D.
facs on lipid accumulation Sorted Low BODIPY Gate +0.2 -0.0 N.D. +1.1 N.D.
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