Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 12047 and 12048 are separated by 165 nucleotides12048 and 12049 are separated by 139 nucleotides12049 and 12050 are separated by 815 nucleotides12050 and 12051 are separated by 272 nucleotides mRNA_3679: 12047 - K02370 EXTL3 alpha-1,4-N-acetylglucosaminyltransferase EXTL3, at 326,327 to 327,835 12047 mRNA_3680: 12048 - Hypothetical Protein, at 328,001 to 330,038 12048 mRNA_3681: 12049 - Hypothetical Protein, at 330,178 to 331,991 12049 mRNA_3682: 12050 - K07203 MTOR, FRAP, TOR serine/threonine-protein kinase mTOR, at 332,807 to 341,262 12050 mRNA_3683: 12051 - KOG0069 Glyoxylate/hydroxypyruvate reductase (D-isomer-specific 2-hydroxy acid dehydrogenase superfamily), at 341,535 to 343,207 12051
Group Condition mRNA_3679 mRNA_3680 mRNA_3681 mRNA_3682 mRNA_3683
12047 12048 12049 12050 12051
facs on lipid accumulation Sorted No BODIPY Gate -0.8 -1.3 -0.1 N.D. +0.0
facs on lipid accumulation Sorted Low BODIPY Gate -1.1 -0.3 -0.5 N.D. -0.1
auxotrophy YNB Glucose -0.1 -1.4 +0.0 N.D. -0.0
growth on ricinoleic acid YNB Methylricinoleic Acid +0.1 -0.8 -0.3 N.D. -0.2
growth on ricinoleic acid YNB Glucose +0.2 -0.6 -0.4 N.D. -0.1
growth on ricinoleic acid YNB Ricinoleic Acid +0.4 -1.0 -0.1 N.D. -0.2
ypd buoyancy 40 hrs No Buoyancy Separation +0.2 -0.7 -0.3 N.D. +0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.3 +0.1 -0.1 N.D. -0.2
auxotrophy Dropout Complete -0.1 -0.5 -0.0 N.D. +0.0
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.3 -0.1 -0.1 N.D. -0.1
facs on lipid accumulation Sorted High BODIPY Gate +0.0 -0.3 -0.1 N.D. -0.2
auxotrophy YNB Glucose 75mM Arginine -0.1 -0.2 +0.0 N.D. -0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.5 +0.1 -0.1 N.D. +0.2
auxotrophy YNB Glucose 75mM Methionine -0.2 -0.1 +0.1 N.D. -0.0
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.2 +0.3 -0.2 N.D. +0.1
growth on oleic acid YNB Oleic Acid -0.0 -0.1 +0.3 N.D. -0.0
growth on oleic acid YNB Glucose +0.1 +0.3 +0.0 N.D. -0.2
ypd buoyancy 40 hrs Median Buoyancy +0.0 +0.4 -0.2 N.D. +0.1
ypd buoyancy 40 hrs Low Buoyancy +0.0 +0.2 -0.0 N.D. +0.2
ypd buoyancy 40 hrs High Buoyancy +0.0 +0.1 +0.2 N.D. +0.2
growth on ypd YPD -0.1 +0.6 +0.4 N.D. -0.0
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