Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 12050 and 12051 are separated by 272 nucleotides12051 and 12052 are separated by 155 nucleotides12052 and 12053 are separated by 365 nucleotides12053 and 12054 are separated by 146 nucleotides mRNA_3682: 12050 - K07203 MTOR, FRAP, TOR serine/threonine-protein kinase mTOR, at 332,807 to 341,262 12050 mRNA_3683: 12051 - KOG0069 Glyoxylate/hydroxypyruvate reductase (D-isomer-specific 2-hydroxy acid dehydrogenase superfamily), at 341,535 to 343,207 12051 mRNA_3684: 12052 - K05955 FNTA protein farnesyltransferase/geranylgeranyltransferase type-1 subunit alpha, at 343,363 to 344,847 12052 mRNA_3685: 12053 - KOG4212 RNA-binding protein hnRNP-M, at 345,213 to 348,082 12053 mRNA_3686: 12054 - SUPERFAMILY--SSF54695, at 348,229 to 349,499 12054
Group Condition mRNA_3682 mRNA_3683 mRNA_3684 mRNA_3685 mRNA_3686
12050 12051 12052 12053 12054
facs on lipid accumulation Sorted Low BODIPY Gate N.D. -0.1 N.D. -1.4 +0.2
facs on lipid accumulation Sorted High BODIPY Gate N.D. -0.2 N.D. -1.0 +0.0
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. -0.2 N.D. -0.9 +0.3
facs on lipid accumulation Sorted No BODIPY Gate N.D. +0.0 N.D. -1.0 +0.2
ypd buoyancy 40 hrs Low Buoyancy N.D. +0.2 N.D. -0.7 -0.2
growth on ypd YPD N.D. -0.0 N.D. -0.3 -0.3
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. -0.2 N.D. -0.1 -0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. +0.1 N.D. -0.8 +0.3
growth on ricinoleic acid YNB Glucose N.D. -0.1 N.D. -0.0 -0.2
growth on oleic acid YNB Glucose N.D. -0.2 N.D. -0.2 +0.0
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. -0.1 N.D. -0.3 +0.1
growth on ricinoleic acid YNB Ricinoleic Acid N.D. -0.2 N.D. -0.0 -0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. +0.2 N.D. -0.6 +0.3
auxotrophy YNB Glucose 75mM Arginine N.D. -0.1 N.D. +0.1 -0.0
auxotrophy YNB Glucose 75mM Methionine N.D. -0.0 N.D. +0.4 -0.4
growth on oleic acid YNB Oleic Acid N.D. -0.0 N.D. -0.1 +0.1
ypd buoyancy 40 hrs Median Buoyancy N.D. +0.1 N.D. +0.0 -0.0
auxotrophy YNB Glucose N.D. -0.0 N.D. +0.2 -0.0
auxotrophy Dropout Complete N.D. +0.0 N.D. +0.1 +0.2
ypd buoyancy 40 hrs High Buoyancy N.D. +0.2 N.D. -0.1 +0.3
ypd buoyancy 40 hrs No Buoyancy Separation N.D. +0.2 N.D. +0.2 +0.2
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