Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 12051 and 12052 are separated by 155 nucleotides12052 and 12053 are separated by 365 nucleotides12053 and 12054 are separated by 146 nucleotides12054 and 12055 are separated by 423 nucleotides mRNA_3683: 12051 - KOG0069 Glyoxylate/hydroxypyruvate reductase (D-isomer-specific 2-hydroxy acid dehydrogenase superfamily), at 341,535 to 343,207 12051 mRNA_3684: 12052 - K05955 FNTA protein farnesyltransferase/geranylgeranyltransferase type-1 subunit alpha, at 343,363 to 344,847 12052 mRNA_3685: 12053 - KOG4212 RNA-binding protein hnRNP-M, at 345,213 to 348,082 12053 mRNA_3686: 12054 - SUPERFAMILY--SSF54695, at 348,229 to 349,499 12054 mRNA_3687: 12055 - K14837 NOP12 nucleolar protein 12, at 349,923 to 352,122 12055
Group Condition mRNA_3683 mRNA_3684 mRNA_3685 mRNA_3686 mRNA_3687
12051 12052 12053 12054 12055
ypd buoyancy 40 hrs No Buoyancy Separation +0.2 N.D. +0.2 +0.2 -2.5
facs on lipid accumulation Sorted High BODIPY Gate -0.2 N.D. -1.0 +0.0 -0.6
facs on lipid accumulation Sorted No BODIPY Gate +0.0 N.D. -1.0 +0.2 -0.9
growth on ypd YPD -0.0 N.D. -0.3 -0.3 -0.7
facs on lipid accumulation Sorted Low BODIPY Gate -0.1 N.D. -1.4 +0.2 +0.4
ypd buoyancy 40 hrs Low Buoyancy +0.2 N.D. -0.7 -0.2 -0.0
growth on oleic acid YNB Glucose -0.2 N.D. -0.2 +0.0 +0.3
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.2 N.D. -0.9 +0.3 +0.7
auxotrophy Dropout Complete +0.0 N.D. +0.1 +0.2 -0.1
auxotrophy YNB Glucose 75mM Arginine -0.1 N.D. +0.1 -0.0 +0.3
auxotrophy YNB Glucose -0.0 N.D. +0.2 -0.0 +0.1
growth on ricinoleic acid YNB Glucose -0.1 N.D. -0.0 -0.2 +0.7
ypd buoyancy 40 hrs Median Buoyancy +0.1 N.D. +0.0 -0.0 +0.4
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.1 N.D. -0.8 +0.3 +1.1
ypd buoyancy 40 hrs High Buoyancy +0.2 N.D. -0.1 +0.3 +0.2
growth on oleic acid YNB Oleic Acid -0.0 N.D. -0.1 +0.1 +0.8
auxotrophy YNB Glucose 75mM Methionine -0.0 N.D. +0.4 -0.4 +0.9
growth on ricinoleic acid YNB Methylricinoleic Acid -0.2 N.D. -0.1 -0.2 +1.4
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.1 N.D. -0.3 +0.1 +1.2
growth on ricinoleic acid YNB Ricinoleic Acid -0.2 N.D. -0.0 -0.1 +1.4
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.2 N.D. -0.6 +0.3 +1.3
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