Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 12052 and 12053 are separated by 365 nucleotides12053 and 12054 are separated by 146 nucleotides12054 and 12055 are separated by 423 nucleotides12055 and 12056 are separated by 160 nucleotides mRNA_3684: 12052 - K05955 FNTA protein farnesyltransferase/geranylgeranyltransferase type-1 subunit alpha, at 343,363 to 344,847 12052 mRNA_3685: 12053 - KOG4212 RNA-binding protein hnRNP-M, at 345,213 to 348,082 12053 mRNA_3686: 12054 - SUPERFAMILY--SSF54695, at 348,229 to 349,499 12054 mRNA_3687: 12055 - K14837 NOP12 nucleolar protein 12, at 349,923 to 352,122 12055 mRNA_3688: 12056 - KOG4285 Mitotic phosphoprotein, at 352,283 to 353,789 12056
Group Condition mRNA_3684 mRNA_3685 mRNA_3686 mRNA_3687 mRNA_3688
12052 12053 12054 12055 12056
facs on lipid accumulation Sorted Low BODIPY Gate N.D. -1.4 +0.2 +0.4 -1.5
ypd buoyancy 40 hrs No Buoyancy Separation N.D. +0.2 +0.2 -2.5 +0.0
facs on lipid accumulation Sorted No BODIPY Gate N.D. -1.0 +0.2 -0.9 -0.0
facs on lipid accumulation Sorted High BODIPY Gate N.D. -1.0 +0.0 -0.6 +0.1
growth on ypd YPD N.D. -0.3 -0.3 -0.7 +0.2
ypd buoyancy 40 hrs Low Buoyancy N.D. -0.7 -0.2 -0.0 +0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. -0.9 +0.3 +0.7 -0.5
growth on ricinoleic acid YNB Glucose N.D. -0.0 -0.2 +0.7 -0.6
growth on oleic acid YNB Glucose N.D. -0.2 +0.0 +0.3 +0.0
ypd buoyancy 40 hrs High Buoyancy N.D. -0.1 +0.3 +0.2 -0.3
auxotrophy Dropout Complete N.D. +0.1 +0.2 -0.1 +0.0
auxotrophy YNB Glucose N.D. +0.2 -0.0 +0.1 -0.0
auxotrophy YNB Glucose 75mM Arginine N.D. +0.1 -0.0 +0.3 -0.0
ypd buoyancy 40 hrs Median Buoyancy N.D. +0.0 -0.0 +0.4 +0.0
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. -0.8 +0.3 +1.1 -0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. -0.6 +0.3 +1.3 -0.4
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. -0.1 -0.2 +1.4 -0.4
growth on oleic acid YNB Oleic Acid N.D. -0.1 +0.1 +0.8 +0.0
auxotrophy YNB Glucose 75mM Methionine N.D. +0.4 -0.4 +0.9 -0.0
growth on ricinoleic acid YNB Ricinoleic Acid N.D. -0.0 -0.1 +1.4 -0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. -0.3 +0.1 +1.2 +0.4
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