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Fitness for 5 genes in
Rhodosporidium toruloides IFO0880
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Top 21 experiments (either direction), sorted by average fitness
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500 nt
12056 and 12057 are separated by 253 nucleotides
12057 and 12058 are separated by 498 nucleotides
12058 and 12059 are separated by 5635 nucleotides
12059 and 12060 are separated by 259 nucleotides
mRNA_3688: 12056 - KOG4285 Mitotic phosphoprotein, at 352,283 to 353,789
12056
mRNA_3689: 12057 - Hypothetical Protein, at 354,043 to 355,679
12057
mRNA_3690: 12058 - BLAST Ser/Thr protein kinase [Mucor ambiguus], at 356,178 to 360,570
12058
mRNA_3691: 12059 - K11839 USP8, UBP5 ubiquitin carboxyl-terminal hydrolase 8, at 366,206 to 370,520
12059
mRNA_3692: 12060 - K04371 MAPK1_3 mitogen-activated protein kinase 1/3, at 370,780 to 372,675
12060
Group
Condition
mRNA
_3688
mRNA
_3689
mRNA
_3690
mRNA
_3691
mRNA
_3692
12056
12057
12058
12059
12060
facs on lipid accumulation
Sorted Low BODIPY Gate
-1.5
N.D.
-0.1
N.D.
-0.9
growth on ricinoleic acid
YNB Ricinoleic Acid
-0.2
N.D.
+0.0
N.D.
-0.5
growth on ricinoleic acid
YNB Glucose
-0.6
N.D.
-0.1
N.D.
+0.0
buoyancy on lipid accumulation
40 hrs No Buoyancy Separation
-0.5
N.D.
-0.2
N.D.
+0.4
growth on ricinoleic acid
YNB Methylricinoleic Acid
-0.4
N.D.
+0.1
N.D.
+0.1
facs on lipid accumulation
Sorted No BODIPY Gate
-0.0
N.D.
+0.1
N.D.
-0.2
growth on oleic acid
YNB Oleic Acid
+0.0
N.D.
-0.2
N.D.
+0.1
auxotrophy
YNB Glucose 75mM Methionine
-0.0
N.D.
-0.1
N.D.
+0.1
ypd buoyancy
40 hrs High Buoyancy
-0.3
N.D.
+0.1
N.D.
+0.2
growth on oleic acid
YNB Glucose
+0.0
N.D.
+0.1
N.D.
-0.2
buoyancy on lipid accumulation
40 hrs High Buoyancy
-0.4
N.D.
+0.1
N.D.
+0.3
auxotrophy
Dropout Complete
+0.0
N.D.
-0.3
N.D.
+0.4
auxotrophy
YNB Glucose 75mM Arginine
-0.0
N.D.
-0.0
N.D.
+0.2
growth on ypd
YPD
+0.2
N.D.
-0.2
N.D.
+0.1
buoyancy on lipid accumulation
40 hrs Median Buoyancy
-0.1
N.D.
+0.1
N.D.
+0.2
buoyancy on lipid accumulation
40 hrs Low Buoyancy
+0.4
N.D.
-0.1
N.D.
+0.0
auxotrophy
YNB Glucose
-0.0
N.D.
-0.2
N.D.
+0.5
facs on lipid accumulation
Sorted High BODIPY Gate
+0.1
N.D.
+0.5
N.D.
-0.3
ypd buoyancy
40 hrs Median Buoyancy
+0.0
N.D.
+0.2
N.D.
+0.3
ypd buoyancy
40 hrs No Buoyancy Separation
+0.0
N.D.
-0.1
N.D.
+0.7
ypd buoyancy
40 hrs Low Buoyancy
+0.2
N.D.
+0.1
N.D.
+0.7
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