Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 12057 and 12058 are separated by 498 nucleotides12058 and 12059 are separated by 5635 nucleotides12059 and 12060 are separated by 259 nucleotides12060 and 12061 are separated by 443 nucleotides mRNA_3689: 12057 - Hypothetical Protein, at 354,043 to 355,679 12057 mRNA_3690: 12058 - BLAST Ser/Thr protein kinase [Mucor ambiguus], at 356,178 to 360,570 12058 mRNA_3691: 12059 - K11839 USP8, UBP5 ubiquitin carboxyl-terminal hydrolase 8, at 366,206 to 370,520 12059 mRNA_3692: 12060 - K04371 MAPK1_3 mitogen-activated protein kinase 1/3, at 370,780 to 372,675 12060 mRNA_3693: 12061 - K01714 dapA 4-hydroxy-tetrahydrodipicolinate synthase, at 373,119 to 374,635 12061
Group Condition mRNA_3689 mRNA_3690 mRNA_3691 mRNA_3692 mRNA_3693
12057 12058 12059 12060 12061
facs on lipid accumulation Sorted Low BODIPY Gate N.D. -0.1 N.D. -0.9 -0.2
growth on oleic acid YNB Oleic Acid N.D. -0.2 N.D. +0.1 -0.7
growth on oleic acid YNB Glucose N.D. +0.1 N.D. -0.2 -0.5
growth on ricinoleic acid YNB Ricinoleic Acid N.D. +0.0 N.D. -0.5 -0.0
growth on ricinoleic acid YNB Glucose N.D. -0.1 N.D. +0.0 -0.4
facs on lipid accumulation Sorted No BODIPY Gate N.D. +0.1 N.D. -0.2 -0.2
auxotrophy YNB Glucose 75mM Methionine N.D. -0.1 N.D. +0.1 -0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. -0.1 N.D. +0.0 -0.0
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. -0.2 N.D. +0.4 -0.2
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. +0.1 N.D. +0.1 -0.2
auxotrophy YNB Glucose 75mM Arginine N.D. -0.0 N.D. +0.2 -0.2
auxotrophy YNB Glucose N.D. -0.2 N.D. +0.5 -0.3
ypd buoyancy 40 hrs High Buoyancy N.D. +0.1 N.D. +0.2 -0.2
auxotrophy Dropout Complete N.D. -0.3 N.D. +0.4 -0.0
facs on lipid accumulation Sorted High BODIPY Gate N.D. +0.5 N.D. -0.3 -0.2
growth on ypd YPD N.D. -0.2 N.D. +0.1 +0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. +0.1 N.D. +0.2 +0.1
ypd buoyancy 40 hrs Median Buoyancy N.D. +0.2 N.D. +0.3 +0.0
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. +0.1 N.D. +0.3 +0.1
ypd buoyancy 40 hrs Low Buoyancy N.D. +0.1 N.D. +0.7 -0.2
ypd buoyancy 40 hrs No Buoyancy Separation N.D. -0.1 N.D. +0.7 +0.2
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