Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 12058 and 12059 are separated by 5635 nucleotides12059 and 12060 are separated by 259 nucleotides12060 and 12061 are separated by 443 nucleotides12061 and 12062 are separated by 1965 nucleotides mRNA_3690: 12058 - BLAST Ser/Thr protein kinase [Mucor ambiguus], at 356,178 to 360,570 12058 mRNA_3691: 12059 - K11839 USP8, UBP5 ubiquitin carboxyl-terminal hydrolase 8, at 366,206 to 370,520 12059 mRNA_3692: 12060 - K04371 MAPK1_3 mitogen-activated protein kinase 1/3, at 370,780 to 372,675 12060 mRNA_3693: 12061 - K01714 dapA 4-hydroxy-tetrahydrodipicolinate synthase, at 373,119 to 374,635 12061 mRNA_3694: 12062 - K18334 fucD L-fuconate dehydratase, at 376,601 to 378,918 12062
Group Condition mRNA_3690 mRNA_3691 mRNA_3692 mRNA_3693 mRNA_3694
12058 12059 12060 12061 12062
facs on lipid accumulation Sorted Low BODIPY Gate -0.1 N.D. -0.9 -0.2 -0.1
growth on oleic acid YNB Oleic Acid -0.2 N.D. +0.1 -0.7 -0.2
growth on ricinoleic acid YNB Glucose -0.1 N.D. +0.0 -0.4 -0.2
growth on ricinoleic acid YNB Ricinoleic Acid +0.0 N.D. -0.5 -0.0 -0.0
growth on oleic acid YNB Glucose +0.1 N.D. -0.2 -0.5 +0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.2 N.D. +0.4 -0.2 -0.5
auxotrophy YNB Glucose 75mM Methionine -0.1 N.D. +0.1 -0.1 -0.3
auxotrophy YNB Glucose -0.2 N.D. +0.5 -0.3 -0.5
facs on lipid accumulation Sorted No BODIPY Gate +0.1 N.D. -0.2 -0.2 -0.1
auxotrophy YNB Glucose 75mM Arginine -0.0 N.D. +0.2 -0.2 -0.4
growth on ricinoleic acid YNB Methylricinoleic Acid +0.1 N.D. +0.1 -0.2 -0.4
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.1 N.D. +0.0 -0.0 -0.2
ypd buoyancy 40 hrs High Buoyancy +0.1 N.D. +0.2 -0.2 -0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.1 N.D. +0.3 +0.1 -0.6
auxotrophy Dropout Complete -0.3 N.D. +0.4 -0.0 -0.2
growth on ypd YPD -0.2 N.D. +0.1 +0.2 -0.2
ypd buoyancy 40 hrs Low Buoyancy +0.1 N.D. +0.7 -0.2 -0.4
facs on lipid accumulation Sorted High BODIPY Gate +0.5 N.D. -0.3 -0.2 +0.0
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.1 N.D. +0.2 +0.1 -0.1
ypd buoyancy 40 hrs Median Buoyancy +0.2 N.D. +0.3 +0.0 -0.1
ypd buoyancy 40 hrs No Buoyancy Separation -0.1 N.D. +0.7 +0.2 -0.0
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