Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 12062 and 12063 are separated by 154 nucleotides12063 and 12064 are separated by 358 nucleotides12064 and 12065 are separated by 274 nucleotides12065 and 12066 are separated by 256 nucleotides mRNA_3694: 12062 - K18334 fucD L-fuconate dehydratase, at 376,601 to 378,918 12062 mRNA_3695: 12063 - KOG1730 Thioredoxin-like protein, at 379,073 to 379,916 12063 mRNA_3696: 12064 - SUPERFAMILY--SSF53335, at 380,275 to 383,085 12064 mRNA_3697: 12065 - K04371 MAPK1_3 mitogen-activated protein kinase 1/3, at 383,360 to 385,326 12065 mRNA_3698: 12066 - K10428 DCTN6 dynactin 6, at 385,583 to 386,475 12066
Group Condition mRNA_3694 mRNA_3695 mRNA_3696 mRNA_3697 mRNA_3698
12062 12063 12064 12065 12066
facs on lipid accumulation Sorted High BODIPY Gate +0.0 -1.0 -0.0 N.D. N.D.
ypd buoyancy 40 hrs No Buoyancy Separation -0.0 -0.7 -0.2 N.D. N.D.
ypd buoyancy 40 hrs Low Buoyancy -0.4 -0.3 -0.0 N.D. N.D.
growth on oleic acid YNB Oleic Acid -0.2 -0.1 -0.3 N.D. N.D.
auxotrophy YNB Glucose -0.5 -0.0 -0.0 N.D. N.D.
growth on oleic acid YNB Glucose +0.1 -0.1 -0.4 N.D. N.D.
facs on lipid accumulation Sorted No BODIPY Gate -0.1 -0.4 +0.2 N.D. N.D.
facs on lipid accumulation Sorted Low BODIPY Gate -0.1 -0.3 +0.0 N.D. N.D.
growth on ricinoleic acid YNB Glucose -0.2 -0.1 +0.1 N.D. N.D.
auxotrophy YNB Glucose 75mM Arginine -0.4 +0.1 +0.1 N.D. N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.5 -0.3 +0.6 N.D. N.D.
auxotrophy YNB Glucose 75mM Methionine -0.3 +0.3 -0.0 N.D. N.D.
ypd buoyancy 40 hrs Median Buoyancy -0.1 -0.1 +0.1 N.D. N.D.
ypd buoyancy 40 hrs High Buoyancy -0.2 -0.2 +0.4 N.D. N.D.
growth on ypd YPD -0.2 +0.1 +0.1 N.D. N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.2 -0.4 +0.8 N.D. N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid -0.4 +0.1 +0.5 N.D. N.D.
auxotrophy Dropout Complete -0.2 +0.2 +0.3 N.D. N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.6 +0.3 +0.8 N.D. N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.1 +0.1 +0.6 N.D. N.D.
growth on ricinoleic acid YNB Ricinoleic Acid -0.0 -0.0 +0.8 N.D. N.D.
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