Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 12064 and 12065 are separated by 274 nucleotides12065 and 12066 are separated by 256 nucleotides12066 and 12067 are separated by 151 nucleotides12067 and 12068 are separated by 278 nucleotides mRNA_3696: 12064 - SUPERFAMILY--SSF53335, at 380,275 to 383,085 12064 mRNA_3697: 12065 - K04371 MAPK1_3 mitogen-activated protein kinase 1/3, at 383,360 to 385,326 12065 mRNA_3698: 12066 - K10428 DCTN6 dynactin 6, at 385,583 to 386,475 12066 mRNA_3699: 12067 - KOG0870 DNA polymerase epsilon, subunit D, at 386,627 to 387,665 12067 mRNA_3700: 12068 - K03020 RPC19, POLR1D DNA-directed RNA polymerases I and III subunit RPAC2, at 387,944 to 388,740 12068
Group Condition mRNA_3696 mRNA_3697 mRNA_3698 mRNA_3699 mRNA_3700
12064 12065 12066 12067 12068
growth on oleic acid YNB Glucose -0.4 N.D. N.D. -0.5 -0.4
ypd buoyancy 40 hrs No Buoyancy Separation -0.2 N.D. N.D. -0.3 -0.9
facs on lipid accumulation Sorted Low BODIPY Gate +0.0 N.D. N.D. -1.6 +0.3
growth on ricinoleic acid YNB Glucose +0.1 N.D. N.D. -0.3 -0.6
auxotrophy Dropout Complete +0.3 N.D. N.D. -0.7 -0.3
auxotrophy YNB Glucose 75mM Methionine -0.0 N.D. N.D. -0.5 -0.1
auxotrophy YNB Glucose -0.0 N.D. N.D. -0.1 -0.1
growth on oleic acid YNB Oleic Acid -0.3 N.D. N.D. +0.1 +0.1
growth on ypd YPD +0.1 N.D. N.D. -0.1 -0.1
facs on lipid accumulation Sorted High BODIPY Gate -0.0 N.D. N.D. -0.4 +0.5
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.8 N.D. N.D. -0.6 -0.1
auxotrophy YNB Glucose 75mM Arginine +0.1 N.D. N.D. -0.1 +0.3
facs on lipid accumulation Sorted No BODIPY Gate +0.2 N.D. N.D. +0.0 +0.1
ypd buoyancy 40 hrs Median Buoyancy +0.1 N.D. N.D. +0.5 -0.2
ypd buoyancy 40 hrs Low Buoyancy -0.0 N.D. N.D. +0.7 -0.2
growth on ricinoleic acid YNB Methylricinoleic Acid +0.5 N.D. N.D. +0.3 -0.3
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.6 N.D. N.D. +0.4 -0.1
ypd buoyancy 40 hrs High Buoyancy +0.4 N.D. N.D. +0.8 +0.1
growth on ricinoleic acid YNB Ricinoleic Acid +0.8 N.D. N.D. +0.3 +0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.8 N.D. N.D. +0.5 +0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.6 N.D. N.D. +1.1 +0.2
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