Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 12065 and 12066 are separated by 256 nucleotides12066 and 12067 are separated by 151 nucleotides12067 and 12068 are separated by 278 nucleotides12068 and 12069 are separated by 348 nucleotides mRNA_3697: 12065 - K04371 MAPK1_3 mitogen-activated protein kinase 1/3, at 383,360 to 385,326 12065 mRNA_3698: 12066 - K10428 DCTN6 dynactin 6, at 385,583 to 386,475 12066 mRNA_3699: 12067 - KOG0870 DNA polymerase epsilon, subunit D, at 386,627 to 387,665 12067 mRNA_3700: 12068 - K03020 RPC19, POLR1D DNA-directed RNA polymerases I and III subunit RPAC2, at 387,944 to 388,740 12068 mRNA_3701: 12069 - ProSiteProfiles-Prokaryotic membrane lipoprotein lipid attachment site profile.-PS51257, at 389,089 to 390,202 12069
Group Condition mRNA_3697 mRNA_3698 mRNA_3699 mRNA_3700 mRNA_3701
12065 12066 12067 12068 12069
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. N.D. -0.6 -0.1 -0.2
facs on lipid accumulation Sorted Low BODIPY Gate N.D. N.D. -1.6 +0.3 +0.3
auxotrophy Dropout Complete N.D. N.D. -0.7 -0.3 +0.2
ypd buoyancy 40 hrs No Buoyancy Separation N.D. N.D. -0.3 -0.9 +0.5
growth on ricinoleic acid YNB Glucose N.D. N.D. -0.3 -0.6 +0.3
auxotrophy YNB Glucose 75mM Methionine N.D. N.D. -0.5 -0.1 +0.3
growth on oleic acid YNB Glucose N.D. N.D. -0.5 -0.4 +0.7
auxotrophy YNB Glucose N.D. N.D. -0.1 -0.1 +0.2
facs on lipid accumulation Sorted High BODIPY Gate N.D. N.D. -0.4 +0.5 +0.0
ypd buoyancy 40 hrs Median Buoyancy N.D. N.D. +0.5 -0.2 -0.1
facs on lipid accumulation Sorted No BODIPY Gate N.D. N.D. +0.0 +0.1 -0.0
growth on ypd YPD N.D. N.D. -0.1 -0.1 +0.3
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. N.D. +0.4 -0.1 -0.1
ypd buoyancy 40 hrs Low Buoyancy N.D. N.D. +0.7 -0.2 +0.0
auxotrophy YNB Glucose 75mM Arginine N.D. N.D. -0.1 +0.3 +0.4
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. N.D. +0.3 -0.3 +0.6
growth on oleic acid YNB Oleic Acid N.D. N.D. +0.1 +0.1 +0.5
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. N.D. +0.5 +0.2 +0.2
ypd buoyancy 40 hrs High Buoyancy N.D. N.D. +0.8 +0.1 +0.2
growth on ricinoleic acid YNB Ricinoleic Acid N.D. N.D. +0.3 +0.2 +0.5
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. N.D. +1.1 +0.2 +0.3
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