Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 12069 and 12070 are separated by 351 nucleotides12070 and 12071 are separated by 230 nucleotides12071 and 12072 are separated by 257 nucleotides12072 and 12073 are separated by 155 nucleotides mRNA_3701: 12069 - ProSiteProfiles-Prokaryotic membrane lipoprotein lipid attachment site profile.-PS51257, at 389,089 to 390,202 12069 mRNA_3702: 12070 - HMMPfam-Inner centromere protein, ARK binding region-PF03941, at 390,554 to 394,764 12070 mRNA_3703: 12071 - HMMPfam-Fungal Zn(2)-Cys(6) binuclear cluster domain-PF00172,HMMPfam-Fungal specific transcription factor domain-PF04082,ProSitePatterns-Zn(2)-C6 fungal-type DNA-binding domain signature.-PS00463,ProSiteProfiles-Zn(2)-C6 fungal-type DNA-binding domain profile.-PS50048,SMART-GAL4-like Zn(II)2Cys6 (or C6 zinc) binuclear cluster DNA-binding domain-SM00066,SUPERFAMILY--SSF57701, at 394,995 to 398,800 12071 mRNA_3704: 12072 - Hypothetical Protein, at 399,058 to 399,573 12072 mRNA_3705: 12073 - Hypothetical Protein, at 399,729 to 400,196 12073
Group Condition mRNA_3701 mRNA_3702 mRNA_3703 mRNA_3704 mRNA_3705
12069 12070 12071 12072 12073
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.2 N.D. -0.7 N.D. +0.1
facs on lipid accumulation Sorted No BODIPY Gate -0.0 N.D. -0.1 N.D. -0.3
growth on ricinoleic acid YNB Ricinoleic Acid +0.5 N.D. -0.3 N.D. -0.4
growth on ypd YPD +0.3 N.D. -0.0 N.D. -0.3
ypd buoyancy 40 hrs Low Buoyancy +0.0 N.D. -0.1 N.D. +0.1
auxotrophy YNB Glucose 75mM Methionine +0.3 N.D. -0.2 N.D. -0.1
ypd buoyancy 40 hrs Median Buoyancy -0.1 N.D. -0.1 N.D. +0.3
facs on lipid accumulation Sorted High BODIPY Gate +0.0 N.D. +0.0 N.D. +0.0
facs on lipid accumulation Sorted Low BODIPY Gate +0.3 N.D. +0.1 N.D. -0.4
growth on oleic acid YNB Oleic Acid +0.5 N.D. -0.2 N.D. -0.3
auxotrophy Dropout Complete +0.2 N.D. -0.2 N.D. +0.1
auxotrophy YNB Glucose +0.2 N.D. -0.2 N.D. +0.1
growth on ricinoleic acid YNB Glucose +0.3 N.D. -0.0 N.D. -0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.1 N.D. -0.0 N.D. +0.3
growth on oleic acid YNB Glucose +0.7 N.D. -0.2 N.D. -0.2
ypd buoyancy 40 hrs High Buoyancy +0.2 N.D. +0.1 N.D. +0.1
growth on ricinoleic acid YNB Methylricinoleic Acid +0.6 N.D. -0.1 N.D. +0.0
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.2 N.D. +0.1 N.D. +0.2
ypd buoyancy 40 hrs No Buoyancy Separation +0.5 N.D. -0.1 N.D. +0.2
auxotrophy YNB Glucose 75mM Arginine +0.4 N.D. -0.1 N.D. +0.3
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.3 N.D. +0.3 N.D. +0.3
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