Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 12070 and 12071 are separated by 230 nucleotides12071 and 12072 are separated by 257 nucleotides12072 and 12073 are separated by 155 nucleotides12073 and 12074 overlap by 11 nucleotides mRNA_3702: 12070 - HMMPfam-Inner centromere protein, ARK binding region-PF03941, at 390,554 to 394,764 12070 mRNA_3703: 12071 - HMMPfam-Fungal Zn(2)-Cys(6) binuclear cluster domain-PF00172,HMMPfam-Fungal specific transcription factor domain-PF04082,ProSitePatterns-Zn(2)-C6 fungal-type DNA-binding domain signature.-PS00463,ProSiteProfiles-Zn(2)-C6 fungal-type DNA-binding domain profile.-PS50048,SMART-GAL4-like Zn(II)2Cys6 (or C6 zinc) binuclear cluster DNA-binding domain-SM00066,SUPERFAMILY--SSF57701, at 394,995 to 398,800 12071 mRNA_3704: 12072 - Hypothetical Protein, at 399,058 to 399,573 12072 mRNA_3705: 12073 - Hypothetical Protein, at 399,729 to 400,196 12073 mRNA_3706: 12074 - KOG1550 Extracellular protein SEL-1 and related proteins, at 400,186 to 402,852 12074
Group Condition mRNA_3702 mRNA_3703 mRNA_3704 mRNA_3705 mRNA_3706
12070 12071 12072 12073 12074
growth on ricinoleic acid YNB Ricinoleic Acid N.D. -0.3 N.D. -0.4 -0.9
growth on oleic acid YNB Glucose N.D. -0.2 N.D. -0.2 -0.5
growth on ricinoleic acid YNB Glucose N.D. -0.0 N.D. -0.1 -0.7
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. -0.7 N.D. +0.1 -0.2
auxotrophy YNB Glucose 75mM Methionine N.D. -0.2 N.D. -0.1 -0.5
facs on lipid accumulation Sorted Low BODIPY Gate N.D. +0.1 N.D. -0.4 -0.5
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. -0.1 N.D. +0.0 -0.6
facs on lipid accumulation Sorted No BODIPY Gate N.D. -0.1 N.D. -0.3 -0.4
auxotrophy YNB Glucose N.D. -0.2 N.D. +0.1 -0.6
growth on ypd YPD N.D. -0.0 N.D. -0.3 -0.2
auxotrophy Dropout Complete N.D. -0.2 N.D. +0.1 -0.0
facs on lipid accumulation Sorted High BODIPY Gate N.D. +0.0 N.D. +0.0 -0.1
growth on oleic acid YNB Oleic Acid N.D. -0.2 N.D. -0.3 +0.4
ypd buoyancy 40 hrs High Buoyancy N.D. +0.1 N.D. +0.1 -0.3
auxotrophy YNB Glucose 75mM Arginine N.D. -0.1 N.D. +0.3 -0.2
ypd buoyancy 40 hrs No Buoyancy Separation N.D. -0.1 N.D. +0.2 -0.1
ypd buoyancy 40 hrs Low Buoyancy N.D. -0.1 N.D. +0.1 +0.1
ypd buoyancy 40 hrs Median Buoyancy N.D. -0.1 N.D. +0.3 -0.0
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. -0.0 N.D. +0.3 -0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. +0.3 N.D. +0.3 -0.0
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. +0.1 N.D. +0.2 +0.3
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