Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 12080 and 12081 are separated by 255 nucleotides12081 and 12082 are separated by 503 nucleotides12082 and 12083 are separated by 234 nucleotides12083 and 12084 are separated by 288 nucleotides mRNA_3712: 12080 - K00003 E1.1.1.3 homoserine dehydrogenase, at 410,154 to 411,574 12080 mRNA_3713: 12081 - KOG4756 Mitochondrial ribosomal protein L27, at 411,830 to 412,320 12081 mRNA_3714: 12082 - K14006 SEC23 protein transport protein SEC23, at 412,824 to 415,636 12082 mRNA_3715: 12083 - K11713 PGTB1 geranylgeranyl transferase type-1 subunit beta, at 415,871 to 417,515 12083 mRNA_3716: 12084 - K13348 MPV17 protein Mpv17, at 417,804 to 418,761 12084
Group Condition mRNA_3712 mRNA_3713 mRNA_3714 mRNA_3715 mRNA_3716
12080 12081 12082 12083 12084
auxotrophy YNB Glucose 75mM Methionine N.D. N.D. N.D. N.D. -1.7
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. N.D. N.D. N.D. -1.3
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. N.D. N.D. N.D. -1.3
facs on lipid accumulation Sorted High BODIPY Gate N.D. N.D. N.D. N.D. -1.0
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. N.D. N.D. N.D. -0.9
auxotrophy YNB Glucose 75mM Arginine N.D. N.D. N.D. N.D. -0.9
facs on lipid accumulation Sorted Low BODIPY Gate N.D. N.D. N.D. N.D. -0.8
auxotrophy YNB Glucose N.D. N.D. N.D. N.D. -0.6
ypd buoyancy 40 hrs No Buoyancy Separation N.D. N.D. N.D. N.D. -0.6
facs on lipid accumulation Sorted No BODIPY Gate N.D. N.D. N.D. N.D. -0.6
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. N.D. N.D. N.D. -0.5
auxotrophy Dropout Complete N.D. N.D. N.D. N.D. -0.4
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. N.D. N.D. N.D. -0.4
growth on oleic acid YNB Glucose N.D. N.D. N.D. N.D. -0.2
ypd buoyancy 40 hrs Median Buoyancy N.D. N.D. N.D. N.D. +0.0
growth on ricinoleic acid YNB Ricinoleic Acid N.D. N.D. N.D. N.D. +0.1
growth on ricinoleic acid YNB Glucose N.D. N.D. N.D. N.D. +0.1
growth on oleic acid YNB Oleic Acid N.D. N.D. N.D. N.D. +0.2
ypd buoyancy 40 hrs High Buoyancy N.D. N.D. N.D. N.D. +0.3
ypd buoyancy 40 hrs Low Buoyancy N.D. N.D. N.D. N.D. +0.3
growth on ypd YPD N.D. N.D. N.D. N.D. +0.7
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