Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 12083 and 12084 are separated by 288 nucleotides12084 and 12085 are separated by 214 nucleotides12085 and 12086 are separated by 280 nucleotides12086 and 12087 are separated by 652 nucleotides mRNA_3715: 12083 - K11713 PGTB1 geranylgeranyl transferase type-1 subunit beta, at 415,871 to 417,515 12083 mRNA_3716: 12084 - K13348 MPV17 protein Mpv17, at 417,804 to 418,761 12084 mRNA_3717: 12085 - K03979 obgE, cgtA GTPase, at 418,976 to 421,073 12085 mRNA_3718: 12086 - K00249 ACADM, acd acyl-CoA dehydrogenase, at 421,354 to 423,944 12086 mRNA_3719: 12087 - K04043 dnaK molecular chaperone DnaK, at 424,597 to 426,828 12087
Group Condition mRNA_3715 mRNA_3716 mRNA_3717 mRNA_3718 mRNA_3719
12083 12084 12085 12086 12087
auxotrophy YNB Glucose 75mM Methionine N.D. -1.7 N.D. -0.0 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. -1.3 N.D. -0.2 N.D.
auxotrophy YNB Glucose 75mM Arginine N.D. -0.9 N.D. -0.4 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. -1.3 N.D. +0.1 N.D.
facs on lipid accumulation Sorted High BODIPY Gate N.D. -1.0 N.D. +0.0 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. -0.9 N.D. +0.0 N.D.
auxotrophy Dropout Complete N.D. -0.4 N.D. -0.4 N.D.
auxotrophy YNB Glucose N.D. -0.6 N.D. -0.2 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. -0.5 N.D. -0.2 N.D.
facs on lipid accumulation Sorted Low BODIPY Gate N.D. -0.8 N.D. +0.3 N.D.
growth on oleic acid YNB Glucose N.D. -0.2 N.D. -0.3 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. -0.4 N.D. -0.1 N.D.
facs on lipid accumulation Sorted No BODIPY Gate N.D. -0.6 N.D. +0.3 N.D.
ypd buoyancy 40 hrs No Buoyancy Separation N.D. -0.6 N.D. +0.4 N.D.
growth on ricinoleic acid YNB Ricinoleic Acid N.D. +0.1 N.D. -0.2 N.D.
ypd buoyancy 40 hrs Median Buoyancy N.D. +0.0 N.D. -0.1 N.D.
ypd buoyancy 40 hrs Low Buoyancy N.D. +0.3 N.D. -0.3 N.D.
growth on oleic acid YNB Oleic Acid N.D. +0.2 N.D. -0.2 N.D.
ypd buoyancy 40 hrs High Buoyancy N.D. +0.3 N.D. -0.1 N.D.
growth on ricinoleic acid YNB Glucose N.D. +0.1 N.D. +0.0 N.D.
growth on ypd YPD N.D. +0.7 N.D. +0.4 N.D.
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