Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 12085 and 12086 are separated by 280 nucleotides12086 and 12087 are separated by 652 nucleotides12087 and 12088 are separated by 267 nucleotides12088 and 12089 are separated by 216 nucleotides mRNA_3717: 12085 - K03979 obgE, cgtA GTPase, at 418,976 to 421,073 12085 mRNA_3718: 12086 - K00249 ACADM, acd acyl-CoA dehydrogenase, at 421,354 to 423,944 12086 mRNA_3719: 12087 - K04043 dnaK molecular chaperone DnaK, at 424,597 to 426,828 12087 mRNA_3720: 12088 - HMMPfam-Conserved hypothetical protein (DUF2461)-PF09365,TIGRFAM-TIGR02453 TIGR02453 family protein-TIGR02453, at 427,096 to 428,543 12088 mRNA_3721: 12089 - K14544 UTP22, NOL6 U3 small nucleolar RNA-associated protein 22, at 428,760 to 433,033 12089
Group Condition mRNA_3717 mRNA_3718 mRNA_3719 mRNA_3720 mRNA_3721
12085 12086 12087 12088 12089
auxotrophy Dropout Complete N.D. -0.4 N.D. -0.2 N.D.
growth on oleic acid YNB Glucose N.D. -0.3 N.D. -0.3 N.D.
auxotrophy YNB Glucose 75mM Arginine N.D. -0.4 N.D. -0.1 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. -0.2 N.D. -0.2 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. +0.1 N.D. -0.4 N.D.
growth on oleic acid YNB Oleic Acid N.D. -0.2 N.D. -0.1 N.D.
ypd buoyancy 40 hrs Low Buoyancy N.D. -0.3 N.D. +0.0 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. -0.2 N.D. -0.0 N.D.
auxotrophy YNB Glucose N.D. -0.2 N.D. +0.0 N.D.
auxotrophy YNB Glucose 75mM Methionine N.D. -0.0 N.D. -0.1 N.D.
ypd buoyancy 40 hrs Median Buoyancy N.D. -0.1 N.D. +0.0 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. -0.1 N.D. +0.0 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. +0.0 N.D. -0.1 N.D.
ypd buoyancy 40 hrs High Buoyancy N.D. -0.1 N.D. +0.1 N.D.
growth on ricinoleic acid YNB Ricinoleic Acid N.D. -0.2 N.D. +0.2 N.D.
facs on lipid accumulation Sorted High BODIPY Gate N.D. +0.0 N.D. +0.1 N.D.
growth on ricinoleic acid YNB Glucose N.D. +0.0 N.D. +0.1 N.D.
facs on lipid accumulation Sorted Low BODIPY Gate N.D. +0.3 N.D. +0.0 N.D.
facs on lipid accumulation Sorted No BODIPY Gate N.D. +0.3 N.D. +0.1 N.D.
growth on ypd YPD N.D. +0.4 N.D. +0.0 N.D.
ypd buoyancy 40 hrs No Buoyancy Separation N.D. +0.4 N.D. +0.1 N.D.
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