Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 12086 and 12087 are separated by 652 nucleotides12087 and 12088 are separated by 267 nucleotides12088 and 12089 are separated by 216 nucleotides12089 and 12090 are separated by 430 nucleotides mRNA_3718: 12086 - K00249 ACADM, acd acyl-CoA dehydrogenase, at 421,354 to 423,944 12086 mRNA_3719: 12087 - K04043 dnaK molecular chaperone DnaK, at 424,597 to 426,828 12087 mRNA_3720: 12088 - HMMPfam-Conserved hypothetical protein (DUF2461)-PF09365,TIGRFAM-TIGR02453 TIGR02453 family protein-TIGR02453, at 427,096 to 428,543 12088 mRNA_3721: 12089 - K14544 UTP22, NOL6 U3 small nucleolar RNA-associated protein 22, at 428,760 to 433,033 12089 mRNA_3722: 12090 - K11251 H2A histone H2A, at 433,464 to 434,074 12090
Group Condition mRNA_3718 mRNA_3719 mRNA_3720 mRNA_3721 mRNA_3722
12086 12087 12088 12089 12090
auxotrophy Dropout Complete -0.4 N.D. -0.2 N.D. -0.6
auxotrophy YNB Glucose 75mM Arginine -0.4 N.D. -0.1 N.D. -0.6
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.2 N.D. -0.2 N.D. -0.5
auxotrophy YNB Glucose 75mM Methionine -0.0 N.D. -0.1 N.D. -0.8
growth on oleic acid YNB Glucose -0.3 N.D. -0.3 N.D. -0.3
growth on oleic acid YNB Oleic Acid -0.2 N.D. -0.1 N.D. -0.3
auxotrophy YNB Glucose -0.2 N.D. +0.0 N.D. -0.3
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.2 N.D. -0.0 N.D. -0.3
ypd buoyancy 40 hrs High Buoyancy -0.1 N.D. +0.1 N.D. -0.4
facs on lipid accumulation Sorted Low BODIPY Gate +0.3 N.D. +0.0 N.D. -0.7
ypd buoyancy 40 hrs Low Buoyancy -0.3 N.D. +0.0 N.D. -0.1
growth on ricinoleic acid YNB Methylricinoleic Acid -0.1 N.D. +0.0 N.D. -0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.1 N.D. -0.4 N.D. +0.1
growth on ricinoleic acid YNB Glucose +0.0 N.D. +0.1 N.D. -0.1
facs on lipid accumulation Sorted High BODIPY Gate +0.0 N.D. +0.1 N.D. -0.1
facs on lipid accumulation Sorted No BODIPY Gate +0.3 N.D. +0.1 N.D. -0.3
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.0 N.D. -0.1 N.D. +0.1
growth on ricinoleic acid YNB Ricinoleic Acid -0.2 N.D. +0.2 N.D. +0.2
growth on ypd YPD +0.4 N.D. +0.0 N.D. -0.0
ypd buoyancy 40 hrs Median Buoyancy -0.1 N.D. +0.0 N.D. +0.6
ypd buoyancy 40 hrs No Buoyancy Separation +0.4 N.D. +0.1 N.D. +1.0
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