Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 12098 and 12099 are separated by 2167 nucleotides12099 and 12100 are separated by 1122 nucleotides12100 and 12101 are separated by 405 nucleotides12101 and 12102 are separated by 482 nucleotides mRNA_3730: 12098 - KOG1268 Glucosamine 6-phosphate synthetases, contain amidotransferase and phosphosugar isomerase domains, at 446,809 to 447,756 12098 mRNA_3731: 12099 - K00820 glmS, GFPT glucosamine--fructose-6-phosphate aminotransferase (isomerizing), at 449,924 to 452,631 12099 mRNA_3732: 12100 - K02541 MCM3 DNA replication licensing factor MCM3, at 453,754 to 456,999 12100 mRNA_3733: 12101 - BLAST Hybrid signal transduction histidine kinase B [Leucoa..., at 457,405 to 460,471 12101 mRNA_3734: 12102 - Interpro Protein HRI1, at 460,954 to 461,748 12102
Group Condition mRNA_3730 mRNA_3731 mRNA_3732 mRNA_3733 mRNA_3734
12098 12099 12100 12101 12102
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. N.D. N.D. -0.4 -0.2
growth on oleic acid YNB Oleic Acid N.D. N.D. N.D. -0.0 -0.4
growth on ricinoleic acid YNB Glucose N.D. N.D. N.D. -0.1 -0.3
growth on ricinoleic acid YNB Ricinoleic Acid N.D. N.D. N.D. -0.3 +0.0
auxotrophy YNB Glucose N.D. N.D. N.D. -0.4 +0.2
growth on ypd YPD N.D. N.D. N.D. -0.1 -0.1
facs on lipid accumulation Sorted High BODIPY Gate N.D. N.D. N.D. -0.2 +0.0
growth on oleic acid YNB Glucose N.D. N.D. N.D. -0.1 -0.0
facs on lipid accumulation Sorted Low BODIPY Gate N.D. N.D. N.D. -0.3 +0.3
auxotrophy YNB Glucose 75mM Arginine N.D. N.D. N.D. -0.3 +0.3
auxotrophy YNB Glucose 75mM Methionine N.D. N.D. N.D. -0.1 +0.1
facs on lipid accumulation Sorted No BODIPY Gate N.D. N.D. N.D. +0.1 -0.0
auxotrophy Dropout Complete N.D. N.D. N.D. -0.2 +0.4
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. N.D. N.D. +0.1 +0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. N.D. N.D. +0.0 +0.4
ypd buoyancy 40 hrs Low Buoyancy N.D. N.D. N.D. -0.0 +0.5
ypd buoyancy 40 hrs Median Buoyancy N.D. N.D. N.D. +0.2 +0.3
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. N.D. N.D. +0.1 +0.5
ypd buoyancy 40 hrs High Buoyancy N.D. N.D. N.D. +0.1 +0.7
ypd buoyancy 40 hrs No Buoyancy Separation N.D. N.D. N.D. +1.0 -0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. N.D. N.D. +0.3 +0.6
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