Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 12099 and 12100 are separated by 1122 nucleotides12100 and 12101 are separated by 405 nucleotides12101 and 12102 are separated by 482 nucleotides12102 and 12103 are separated by 152 nucleotides mRNA_3731: 12099 - K00820 glmS, GFPT glucosamine--fructose-6-phosphate aminotransferase (isomerizing), at 449,924 to 452,631 12099 mRNA_3732: 12100 - K02541 MCM3 DNA replication licensing factor MCM3, at 453,754 to 456,999 12100 mRNA_3733: 12101 - BLAST Hybrid signal transduction histidine kinase B [Leucoa..., at 457,405 to 460,471 12101 mRNA_3734: 12102 - Interpro Protein HRI1, at 460,954 to 461,748 12102 mRNA_3735: 12103 - KOG4842 Protein involved in sister chromatid separation and/or segregation, at 461,901 to 463,089 12103
Group Condition mRNA_3731 mRNA_3732 mRNA_3733 mRNA_3734 mRNA_3735
12099 12100 12101 12102 12103
growth on ypd YPD N.D. N.D. -0.1 -0.1 -0.7
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. N.D. -0.4 -0.2 -0.3
facs on lipid accumulation Sorted High BODIPY Gate N.D. N.D. -0.2 +0.0 -0.7
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. N.D. +0.1 +0.2 -1.0
facs on lipid accumulation Sorted No BODIPY Gate N.D. N.D. +0.1 -0.0 -0.5
facs on lipid accumulation Sorted Low BODIPY Gate N.D. N.D. -0.3 +0.3 -0.3
auxotrophy YNB Glucose 75mM Methionine N.D. N.D. -0.1 +0.1 -0.2
growth on oleic acid YNB Oleic Acid N.D. N.D. -0.0 -0.4 +0.3
auxotrophy YNB Glucose N.D. N.D. -0.4 +0.2 +0.3
growth on oleic acid YNB Glucose N.D. N.D. -0.1 -0.0 +0.2
growth on ricinoleic acid YNB Glucose N.D. N.D. -0.1 -0.3 +0.6
growth on ricinoleic acid YNB Ricinoleic Acid N.D. N.D. -0.3 +0.0 +0.5
auxotrophy YNB Glucose 75mM Arginine N.D. N.D. -0.3 +0.3 +0.3
ypd buoyancy 40 hrs High Buoyancy N.D. N.D. +0.1 +0.7 -0.3
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. N.D. +0.1 +0.5 -0.0
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. N.D. +0.0 +0.4 +0.4
ypd buoyancy 40 hrs Low Buoyancy N.D. N.D. -0.0 +0.5 +0.3
ypd buoyancy 40 hrs No Buoyancy Separation N.D. N.D. +1.0 -0.2 +0.1
ypd buoyancy 40 hrs Median Buoyancy N.D. N.D. +0.2 +0.3 +0.4
auxotrophy Dropout Complete N.D. N.D. -0.2 +0.4 +0.7
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. N.D. +0.3 +0.6 +0.3
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