Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 12116 and 12117 are separated by 377 nucleotides12117 and 12118 are separated by 84 nucleotides12118 and 12119 are separated by 227 nucleotides12119 and 12120 are separated by 190 nucleotides mRNA_3748: 12116 - K00658 DLST, sucB 2-oxoglutarate dehydrogenase E2 component (dihydrolipoamide succinyltransferase), at 490,464 to 492,382 12116 mRNA_3749: 12117 - HMMPfam-Nuclear pore complex assembly-PF13934,SUPERFAMILY--SSF57850, at 492,760 to 497,695 12117 mRNA_3750: 12118 - K13421 UMPS uridine monophosphate synthetase, at 497,780 to 498,794 12118 mRNA_3751: 12119 - K11348 BRPF1 bromodomain and PHD finger-containing protein 1, at 499,022 to 503,388 12119 mRNA_3752: 12120 - HMMPfam-GDP-fucose protein O-fucosyltransferase-PF10250, at 503,579 to 505,478 12120
Group Condition mRNA_3748 mRNA_3749 mRNA_3750 mRNA_3751 mRNA_3752
12116 12117 12118 12119 12120
facs on lipid accumulation Sorted Low BODIPY Gate N.D. -0.6 N.D. -0.1 -0.2
facs on lipid accumulation Sorted High BODIPY Gate N.D. -0.7 N.D. -0.4 +0.2
auxotrophy Dropout Complete N.D. -0.2 N.D. -0.3 +0.1
auxotrophy YNB Glucose N.D. -0.3 N.D. -0.2 +0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. +0.0 N.D. -0.4 +0.0
auxotrophy YNB Glucose 75mM Arginine N.D. -0.2 N.D. -0.1 +0.1
auxotrophy YNB Glucose 75mM Methionine N.D. -0.2 N.D. -0.0 -0.0
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. -0.3 N.D. +0.0 +0.1
facs on lipid accumulation Sorted No BODIPY Gate N.D. -0.2 N.D. -0.4 +0.4
growth on ypd YPD N.D. +0.0 N.D. +0.1 -0.1
growth on oleic acid YNB Oleic Acid N.D. -0.2 N.D. +0.1 +0.1
ypd buoyancy 40 hrs Low Buoyancy N.D. -0.1 N.D. -0.2 +0.3
growth on ricinoleic acid YNB Glucose N.D. -0.2 N.D. -0.0 +0.3
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. -0.2 N.D. -0.0 +0.3
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. +0.0 N.D. -0.0 +0.1
growth on oleic acid YNB Glucose N.D. -0.2 N.D. +0.2 +0.1
growth on ricinoleic acid YNB Ricinoleic Acid N.D. -0.3 N.D. -0.2 +0.6
ypd buoyancy 40 hrs Median Buoyancy N.D. -0.0 N.D. -0.1 +0.3
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. -0.1 N.D. +0.3 +0.1
ypd buoyancy 40 hrs No Buoyancy Separation N.D. +0.1 N.D. +0.1 +0.2
ypd buoyancy 40 hrs High Buoyancy N.D. +0.0 N.D. +0.1 +0.3
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