Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 12120 and 12121 are separated by 176 nucleotides12121 and 12122 are separated by 319 nucleotides12122 and 12123 are separated by 334 nucleotides12123 and 12124 are separated by 72 nucleotides mRNA_3752: 12120 - HMMPfam-GDP-fucose protein O-fucosyltransferase-PF10250, at 503,579 to 505,478 12120 mRNA_3753: 12121 - K00728 POMT dolichyl-phosphate-mannose-protein mannosyltransferase, at 505,655 to 508,720 12121 mRNA_3754: 12122 - K01641 E2.3.3.10 hydroxymethylglutaryl-CoA synthase, at 509,040 to 510,998 12122 mRNA_3755: 12123 - K01893 NARS, asnS asparaginyl-tRNA synthetase, at 511,333 to 513,065 12123 mRNA_3756: 12124 - HMMPfam-Protein of unknown function (DUF2665)-PF11654, at 513,138 to 513,518 12124
Group Condition mRNA_3752 mRNA_3753 mRNA_3754 mRNA_3755 mRNA_3756
12120 12121 12122 12123 12124
facs on lipid accumulation Sorted Low BODIPY Gate -0.2 -3.7 N.D. N.D. -0.6
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.1 -1.7 N.D. N.D. -0.7
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.3 -0.8 N.D. N.D. -0.5
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.1 -0.2 N.D. N.D. -0.9
ypd buoyancy 40 hrs No Buoyancy Separation +0.2 -0.7 N.D. N.D. -0.2
ypd buoyancy 40 hrs High Buoyancy +0.3 -0.5 N.D. N.D. -0.3
facs on lipid accumulation Sorted No BODIPY Gate +0.4 -0.9 N.D. N.D. +0.1
ypd buoyancy 40 hrs Median Buoyancy +0.3 -0.9 N.D. N.D. +0.6
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.0 +0.3 N.D. N.D. -0.1
auxotrophy YNB Glucose 75mM Arginine +0.1 +0.5 N.D. N.D. -0.3
auxotrophy YNB Glucose 75mM Methionine -0.0 +0.5 N.D. N.D. +0.1
growth on oleic acid YNB Glucose +0.1 +1.0 N.D. N.D. +0.1
auxotrophy Dropout Complete +0.1 +0.8 N.D. N.D. +0.3
ypd buoyancy 40 hrs Low Buoyancy +0.3 +1.5 N.D. N.D. -0.6
growth on oleic acid YNB Oleic Acid +0.1 +1.0 N.D. N.D. +0.3
growth on ricinoleic acid YNB Glucose +0.3 +1.3 N.D. N.D. -0.1
auxotrophy YNB Glucose +0.1 +0.9 N.D. N.D. +0.6
growth on ypd YPD -0.1 +0.2 N.D. N.D. +1.9
growth on ricinoleic acid YNB Ricinoleic Acid +0.6 +2.0 N.D. N.D. -0.6
facs on lipid accumulation Sorted High BODIPY Gate +0.2 +1.7 N.D. N.D. +0.5
growth on ricinoleic acid YNB Methylricinoleic Acid +0.1 +2.5 N.D. N.D. +0.1
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