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Fitness for 5 genes in
Rhodosporidium toruloides IFO0880
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Top 21 experiments (either direction), sorted by average fitness
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500 nt
12120 and 12121 are separated by 176 nucleotides
12121 and 12122 are separated by 319 nucleotides
12122 and 12123 are separated by 334 nucleotides
12123 and 12124 are separated by 72 nucleotides
mRNA_3752: 12120 - HMMPfam-GDP-fucose protein O-fucosyltransferase-PF10250, at 503,579 to 505,478
12120
mRNA_3753: 12121 - K00728 POMT dolichyl-phosphate-mannose-protein mannosyltransferase, at 505,655 to 508,720
12121
mRNA_3754: 12122 - K01641 E2.3.3.10 hydroxymethylglutaryl-CoA synthase, at 509,040 to 510,998
12122
mRNA_3755: 12123 - K01893 NARS, asnS asparaginyl-tRNA synthetase, at 511,333 to 513,065
12123
mRNA_3756: 12124 - HMMPfam-Protein of unknown function (DUF2665)-PF11654, at 513,138 to 513,518
12124
Group
Condition
mRNA
_3752
mRNA
_3753
mRNA
_3754
mRNA
_3755
mRNA
_3756
12120
12121
12122
12123
12124
facs on lipid accumulation
Sorted Low BODIPY Gate
-0.2
-3.7
N.D.
N.D.
-0.6
buoyancy on lipid accumulation
40 hrs High Buoyancy
+0.1
-1.7
N.D.
N.D.
-0.7
buoyancy on lipid accumulation
40 hrs Median Buoyancy
+0.3
-0.8
N.D.
N.D.
-0.5
buoyancy on lipid accumulation
40 hrs No Buoyancy Separation
+0.1
-0.2
N.D.
N.D.
-0.9
ypd buoyancy
40 hrs No Buoyancy Separation
+0.2
-0.7
N.D.
N.D.
-0.2
ypd buoyancy
40 hrs High Buoyancy
+0.3
-0.5
N.D.
N.D.
-0.3
facs on lipid accumulation
Sorted No BODIPY Gate
+0.4
-0.9
N.D.
N.D.
+0.1
ypd buoyancy
40 hrs Median Buoyancy
+0.3
-0.9
N.D.
N.D.
+0.6
buoyancy on lipid accumulation
40 hrs Low Buoyancy
+0.0
+0.3
N.D.
N.D.
-0.1
auxotrophy
YNB Glucose 75mM Arginine
+0.1
+0.5
N.D.
N.D.
-0.3
auxotrophy
YNB Glucose 75mM Methionine
-0.0
+0.5
N.D.
N.D.
+0.1
growth on oleic acid
YNB Glucose
+0.1
+1.0
N.D.
N.D.
+0.1
auxotrophy
Dropout Complete
+0.1
+0.8
N.D.
N.D.
+0.3
ypd buoyancy
40 hrs Low Buoyancy
+0.3
+1.5
N.D.
N.D.
-0.6
growth on oleic acid
YNB Oleic Acid
+0.1
+1.0
N.D.
N.D.
+0.3
growth on ricinoleic acid
YNB Glucose
+0.3
+1.3
N.D.
N.D.
-0.1
auxotrophy
YNB Glucose
+0.1
+0.9
N.D.
N.D.
+0.6
growth on ypd
YPD
-0.1
+0.2
N.D.
N.D.
+1.9
growth on ricinoleic acid
YNB Ricinoleic Acid
+0.6
+2.0
N.D.
N.D.
-0.6
facs on lipid accumulation
Sorted High BODIPY Gate
+0.2
+1.7
N.D.
N.D.
+0.5
growth on ricinoleic acid
YNB Methylricinoleic Acid
+0.1
+2.5
N.D.
N.D.
+0.1
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