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Fitness for 5 genes in
Rhodosporidium toruloides IFO0880
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Top 21 experiments (either direction), sorted by average fitness
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500 nt
12131 and 12132 are separated by 155 nucleotides
12132 and 12133 are separated by 154 nucleotides
12133 and 12134 are separated by 100 nucleotides
12134 and 12135 are separated by 155 nucleotides
mRNA_3763: 12131 - Hypothetical Protein, at 526,166 to 531,244
12131
mRNA_3764: 12132 - K11808 ADE2 phosphoribosylaminoimidazole carboxylase, at 531,400 to 533,650
12132
mRNA_3765: 12133 - K02902 RP-L28, MRPL28, rpmB large subunit ribosomal protein L28, at 533,805 to 534,326
12133
mRNA_3766: 12134 - K20372 TBC1D20, GYP8 TBC1 domain family member 20, at 534,427 to 536,780
12134
mRNA_3767: 12135 - KOG4535 HEAT and armadillo repeat-containing protein, at 536,936 to 539,895
12135
Group
Condition
mRNA
_3763
mRNA
_3764
mRNA
_3765
mRNA
_3766
mRNA
_3767
12131
12132
12133
12134
12135
ypd buoyancy
40 hrs No Buoyancy Separation
-0.4
N.D.
N.D.
-0.5
-0.7
facs on lipid accumulation
Sorted Low BODIPY Gate
-0.2
N.D.
N.D.
-0.1
-0.2
ypd buoyancy
40 hrs Low Buoyancy
-0.1
N.D.
N.D.
-0.1
-0.1
facs on lipid accumulation
Sorted No BODIPY Gate
-0.0
N.D.
N.D.
-0.2
-0.1
growth on ricinoleic acid
YNB Ricinoleic Acid
-0.4
N.D.
N.D.
-0.1
+0.2
growth on ricinoleic acid
YNB Methylricinoleic Acid
-0.3
N.D.
N.D.
+0.1
-0.0
buoyancy on lipid accumulation
40 hrs Low Buoyancy
-0.2
N.D.
N.D.
+0.2
-0.1
auxotrophy
YNB Glucose 75mM Arginine
-0.2
N.D.
N.D.
+0.2
-0.1
growth on oleic acid
YNB Oleic Acid
-0.5
N.D.
N.D.
+0.3
+0.1
buoyancy on lipid accumulation
40 hrs No Buoyancy Separation
-0.1
N.D.
N.D.
+0.1
-0.1
facs on lipid accumulation
Sorted High BODIPY Gate
+0.0
N.D.
N.D.
+0.0
-0.1
buoyancy on lipid accumulation
40 hrs Median Buoyancy
+0.1
N.D.
N.D.
+0.1
-0.2
auxotrophy
YNB Glucose 75mM Methionine
-0.3
N.D.
N.D.
+0.4
-0.1
ypd buoyancy
40 hrs High Buoyancy
+0.1
N.D.
N.D.
-0.2
+0.2
growth on ricinoleic acid
YNB Glucose
+0.0
N.D.
N.D.
+0.1
-0.0
ypd buoyancy
40 hrs Median Buoyancy
+0.3
N.D.
N.D.
-0.0
-0.1
buoyancy on lipid accumulation
40 hrs High Buoyancy
+0.0
N.D.
N.D.
+0.2
-0.1
auxotrophy
YNB Glucose
-0.2
N.D.
N.D.
+0.1
+0.3
growth on oleic acid
YNB Glucose
-0.2
N.D.
N.D.
+0.3
+0.1
auxotrophy
Dropout Complete
-0.3
N.D.
N.D.
+0.4
+0.1
growth on ypd
YPD
+0.0
N.D.
N.D.
-0.4
+0.6
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