Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 12131 and 12132 are separated by 155 nucleotides12132 and 12133 are separated by 154 nucleotides12133 and 12134 are separated by 100 nucleotides12134 and 12135 are separated by 155 nucleotides mRNA_3763: 12131 - Hypothetical Protein, at 526,166 to 531,244 12131 mRNA_3764: 12132 - K11808 ADE2 phosphoribosylaminoimidazole carboxylase, at 531,400 to 533,650 12132 mRNA_3765: 12133 - K02902 RP-L28, MRPL28, rpmB large subunit ribosomal protein L28, at 533,805 to 534,326 12133 mRNA_3766: 12134 - K20372 TBC1D20, GYP8 TBC1 domain family member 20, at 534,427 to 536,780 12134 mRNA_3767: 12135 - KOG4535 HEAT and armadillo repeat-containing protein, at 536,936 to 539,895 12135
Group Condition mRNA_3763 mRNA_3764 mRNA_3765 mRNA_3766 mRNA_3767
12131 12132 12133 12134 12135
ypd buoyancy 40 hrs No Buoyancy Separation -0.4 N.D. N.D. -0.5 -0.7
facs on lipid accumulation Sorted Low BODIPY Gate -0.2 N.D. N.D. -0.1 -0.2
ypd buoyancy 40 hrs Low Buoyancy -0.1 N.D. N.D. -0.1 -0.1
facs on lipid accumulation Sorted No BODIPY Gate -0.0 N.D. N.D. -0.2 -0.1
growth on ricinoleic acid YNB Ricinoleic Acid -0.4 N.D. N.D. -0.1 +0.2
growth on ricinoleic acid YNB Methylricinoleic Acid -0.3 N.D. N.D. +0.1 -0.0
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.2 N.D. N.D. +0.2 -0.1
auxotrophy YNB Glucose 75mM Arginine -0.2 N.D. N.D. +0.2 -0.1
growth on oleic acid YNB Oleic Acid -0.5 N.D. N.D. +0.3 +0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.1 N.D. N.D. +0.1 -0.1
facs on lipid accumulation Sorted High BODIPY Gate +0.0 N.D. N.D. +0.0 -0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.1 N.D. N.D. +0.1 -0.2
auxotrophy YNB Glucose 75mM Methionine -0.3 N.D. N.D. +0.4 -0.1
ypd buoyancy 40 hrs High Buoyancy +0.1 N.D. N.D. -0.2 +0.2
growth on ricinoleic acid YNB Glucose +0.0 N.D. N.D. +0.1 -0.0
ypd buoyancy 40 hrs Median Buoyancy +0.3 N.D. N.D. -0.0 -0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.0 N.D. N.D. +0.2 -0.1
auxotrophy YNB Glucose -0.2 N.D. N.D. +0.1 +0.3
growth on oleic acid YNB Glucose -0.2 N.D. N.D. +0.3 +0.1
auxotrophy Dropout Complete -0.3 N.D. N.D. +0.4 +0.1
growth on ypd YPD +0.0 N.D. N.D. -0.4 +0.6
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