Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 12132 and 12133 are separated by 154 nucleotides12133 and 12134 are separated by 100 nucleotides12134 and 12135 are separated by 155 nucleotides12135 and 12136 are separated by 173 nucleotides mRNA_3764: 12132 - K11808 ADE2 phosphoribosylaminoimidazole carboxylase, at 531,400 to 533,650 12132 mRNA_3765: 12133 - K02902 RP-L28, MRPL28, rpmB large subunit ribosomal protein L28, at 533,805 to 534,326 12133 mRNA_3766: 12134 - K20372 TBC1D20, GYP8 TBC1 domain family member 20, at 534,427 to 536,780 12134 mRNA_3767: 12135 - KOG4535 HEAT and armadillo repeat-containing protein, at 536,936 to 539,895 12135 mRNA_3768: 12136 - HMMPfam-Protein of unknown function (DUF1275)-PF06912, at 540,069 to 541,238 12136
Group Condition mRNA_3764 mRNA_3765 mRNA_3766 mRNA_3767 mRNA_3768
12132 12133 12134 12135 12136
ypd buoyancy 40 hrs No Buoyancy Separation N.D. N.D. -0.5 -0.7 +0.7
growth on ricinoleic acid YNB Glucose N.D. N.D. +0.1 -0.0 -0.4
ypd buoyancy 40 hrs Low Buoyancy N.D. N.D. -0.1 -0.1 -0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. N.D. +0.1 -0.1 -0.3
growth on ricinoleic acid YNB Ricinoleic Acid N.D. N.D. -0.1 +0.2 -0.4
growth on oleic acid YNB Oleic Acid N.D. N.D. +0.3 +0.1 -0.6
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. N.D. +0.1 -0.0 -0.2
ypd buoyancy 40 hrs Median Buoyancy N.D. N.D. -0.0 -0.1 +0.1
growth on ypd YPD N.D. N.D. -0.4 +0.6 -0.3
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. N.D. +0.2 -0.1 -0.0
facs on lipid accumulation Sorted High BODIPY Gate N.D. N.D. +0.0 -0.1 +0.2
ypd buoyancy 40 hrs High Buoyancy N.D. N.D. -0.2 +0.2 +0.1
facs on lipid accumulation Sorted No BODIPY Gate N.D. N.D. -0.2 -0.1 +0.4
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. N.D. +0.1 -0.2 +0.2
growth on oleic acid YNB Glucose N.D. N.D. +0.3 +0.1 -0.2
facs on lipid accumulation Sorted Low BODIPY Gate N.D. N.D. -0.1 -0.2 +0.4
auxotrophy Dropout Complete N.D. N.D. +0.4 +0.1 -0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. N.D. +0.2 -0.1 +0.2
auxotrophy YNB Glucose 75mM Arginine N.D. N.D. +0.2 -0.1 +0.3
auxotrophy YNB Glucose 75mM Methionine N.D. N.D. +0.4 -0.1 +0.1
auxotrophy YNB Glucose N.D. N.D. +0.1 +0.3 +0.1
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