Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 12134 and 12135 are separated by 155 nucleotides12135 and 12136 are separated by 173 nucleotides12136 and 12137 are separated by 195 nucleotides12137 and 12138 are separated by 303 nucleotides mRNA_3766: 12134 - K20372 TBC1D20, GYP8 TBC1 domain family member 20, at 534,427 to 536,780 12134 mRNA_3767: 12135 - KOG4535 HEAT and armadillo repeat-containing protein, at 536,936 to 539,895 12135 mRNA_3768: 12136 - HMMPfam-Protein of unknown function (DUF1275)-PF06912, at 540,069 to 541,238 12136 mRNA_3769: 12137 - K10669 TRPT1, TPT1 2'-phosphotransferase, at 541,434 to 542,462 12137 mRNA_3770: 12138 - K12606 RCD1, CNOT9, CAF40 CCR4-NOT transcription complex subunit 9, at 542,766 to 544,325 12138
Group Condition mRNA_3766 mRNA_3767 mRNA_3768 mRNA_3769 mRNA_3770
12134 12135 12136 12137 12138
ypd buoyancy 40 hrs No Buoyancy Separation -0.5 -0.7 +0.7 N.D. -0.4
growth on ricinoleic acid YNB Glucose +0.1 -0.0 -0.4 N.D. -0.0
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.1 -0.1 -0.3 N.D. +0.1
growth on oleic acid YNB Glucose +0.3 +0.1 -0.2 N.D. -0.4
facs on lipid accumulation Sorted High BODIPY Gate +0.0 -0.1 +0.2 N.D. -0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.2 -0.1 +0.2 N.D. -0.4
growth on ricinoleic acid YNB Methylricinoleic Acid +0.1 -0.0 -0.2 N.D. +0.0
growth on ypd YPD -0.4 +0.6 -0.3 N.D. +0.0
ypd buoyancy 40 hrs Median Buoyancy -0.0 -0.1 +0.1 N.D. +0.1
auxotrophy Dropout Complete +0.4 +0.1 -0.2 N.D. +0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.2 -0.1 -0.0 N.D. +0.3
auxotrophy YNB Glucose 75mM Methionine +0.4 -0.1 +0.1 N.D. +0.2
ypd buoyancy 40 hrs High Buoyancy -0.2 +0.2 +0.1 N.D. +0.5
auxotrophy YNB Glucose 75mM Arginine +0.2 -0.1 +0.3 N.D. +0.2
ypd buoyancy 40 hrs Low Buoyancy -0.1 -0.1 -0.1 N.D. +1.0
facs on lipid accumulation Sorted No BODIPY Gate -0.2 -0.1 +0.4 N.D. +0.6
auxotrophy YNB Glucose +0.1 +0.3 +0.1 N.D. +0.3
growth on oleic acid YNB Oleic Acid +0.3 +0.1 -0.6 N.D. +1.0
growth on ricinoleic acid YNB Ricinoleic Acid -0.1 +0.2 -0.4 N.D. +1.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.1 -0.2 +0.2 N.D. +0.9
facs on lipid accumulation Sorted Low BODIPY Gate -0.1 -0.2 +0.4 N.D. +0.9
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