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Fitness for 5 genes in
Rhodosporidium toruloides IFO0880
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Top 21 experiments (either direction), sorted by average fitness
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500 nt
12134 and 12135 are separated by 155 nucleotides
12135 and 12136 are separated by 173 nucleotides
12136 and 12137 are separated by 195 nucleotides
12137 and 12138 are separated by 303 nucleotides
mRNA_3766: 12134 - K20372 TBC1D20, GYP8 TBC1 domain family member 20, at 534,427 to 536,780
12134
mRNA_3767: 12135 - KOG4535 HEAT and armadillo repeat-containing protein, at 536,936 to 539,895
12135
mRNA_3768: 12136 - HMMPfam-Protein of unknown function (DUF1275)-PF06912, at 540,069 to 541,238
12136
mRNA_3769: 12137 - K10669 TRPT1, TPT1 2'-phosphotransferase, at 541,434 to 542,462
12137
mRNA_3770: 12138 - K12606 RCD1, CNOT9, CAF40 CCR4-NOT transcription complex subunit 9, at 542,766 to 544,325
12138
Group
Condition
mRNA
_3766
mRNA
_3767
mRNA
_3768
mRNA
_3769
mRNA
_3770
12134
12135
12136
12137
12138
ypd buoyancy
40 hrs No Buoyancy Separation
-0.5
-0.7
+0.7
N.D.
-0.4
growth on ricinoleic acid
YNB Glucose
+0.1
-0.0
-0.4
N.D.
-0.0
buoyancy on lipid accumulation
40 hrs No Buoyancy Separation
+0.1
-0.1
-0.3
N.D.
+0.1
growth on oleic acid
YNB Glucose
+0.3
+0.1
-0.2
N.D.
-0.4
facs on lipid accumulation
Sorted High BODIPY Gate
+0.0
-0.1
+0.2
N.D.
-0.2
buoyancy on lipid accumulation
40 hrs Low Buoyancy
+0.2
-0.1
+0.2
N.D.
-0.4
growth on ricinoleic acid
YNB Methylricinoleic Acid
+0.1
-0.0
-0.2
N.D.
+0.0
growth on ypd
YPD
-0.4
+0.6
-0.3
N.D.
+0.0
ypd buoyancy
40 hrs Median Buoyancy
-0.0
-0.1
+0.1
N.D.
+0.1
auxotrophy
Dropout Complete
+0.4
+0.1
-0.2
N.D.
+0.1
buoyancy on lipid accumulation
40 hrs High Buoyancy
+0.2
-0.1
-0.0
N.D.
+0.3
auxotrophy
YNB Glucose 75mM Methionine
+0.4
-0.1
+0.1
N.D.
+0.2
ypd buoyancy
40 hrs High Buoyancy
-0.2
+0.2
+0.1
N.D.
+0.5
auxotrophy
YNB Glucose 75mM Arginine
+0.2
-0.1
+0.3
N.D.
+0.2
ypd buoyancy
40 hrs Low Buoyancy
-0.1
-0.1
-0.1
N.D.
+1.0
facs on lipid accumulation
Sorted No BODIPY Gate
-0.2
-0.1
+0.4
N.D.
+0.6
auxotrophy
YNB Glucose
+0.1
+0.3
+0.1
N.D.
+0.3
growth on oleic acid
YNB Oleic Acid
+0.3
+0.1
-0.6
N.D.
+1.0
growth on ricinoleic acid
YNB Ricinoleic Acid
-0.1
+0.2
-0.4
N.D.
+1.2
buoyancy on lipid accumulation
40 hrs Median Buoyancy
+0.1
-0.2
+0.2
N.D.
+0.9
facs on lipid accumulation
Sorted Low BODIPY Gate
-0.1
-0.2
+0.4
N.D.
+0.9
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