Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 12144 and 12145 are separated by 605 nucleotides12145 and 12146 are separated by 450 nucleotides12146 and 12147 are separated by 774 nucleotides12147 and 12148 are separated by 246 nucleotides mRNA_3776: 12144 - HMMPfam-FHA domain-PF00498,HMMPfam-Ring finger domain-PF13639,ProSiteProfiles-Forkhead-associated (FHA) domain profile.-PS50006,ProSiteProfiles-Zinc finger RING-type profile.-PS50089,SMART-Ring finger-SM00184,SMART-Forkhead associated domain-SM00240,SUPERFAMILY--SSF49879,SUPERFAMILY--SSF57850, at 553,955 to 556,574 12144 mRNA_3777: 12145 - K20346 TMED4_9_11 p24 family protein alpha, at 557,180 to 558,034 12145 mRNA_3778: 12146 - HMMPfam-Endonuclease V-PF04493, at 558,485 to 559,797 12146 mRNA_3779: 12147 - HMMPfam-Spc19-PF08287, at 560,572 to 561,347 12147 mRNA_3780: 12148 - KOG1426 FOG RCC1 domain, at 561,594 to 563,970 12148
Group Condition mRNA_3776 mRNA_3777 mRNA_3778 mRNA_3779 mRNA_3780
12144 12145 12146 12147 12148
facs on lipid accumulation Sorted Low BODIPY Gate -1.1 -2.1 -0.7 N.D. -0.3
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.2 -1.7 +0.2 N.D. -0.1
facs on lipid accumulation Sorted No BODIPY Gate -0.2 -0.9 -0.2 N.D. -0.2
growth on ypd YPD -0.3 +0.0 -0.3 N.D. -0.0
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.5 +0.0 +0.1 N.D. -0.0
growth on ricinoleic acid YNB Methylricinoleic Acid -0.8 +0.7 -0.6 N.D. +0.3
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.9 -0.0 +0.6 N.D. +0.0
ypd buoyancy 40 hrs Median Buoyancy -0.0 -0.0 -0.3 N.D. +0.1
ypd buoyancy 40 hrs High Buoyancy -0.5 +0.4 -0.1 N.D. -0.0
growth on oleic acid YNB Oleic Acid -1.2 +0.8 +0.1 N.D. +0.1
ypd buoyancy 40 hrs No Buoyancy Separation +0.1 +0.5 +0.1 N.D. -0.8
ypd buoyancy 40 hrs Low Buoyancy -0.6 +1.0 -0.4 N.D. +0.1
facs on lipid accumulation Sorted High BODIPY Gate -0.2 +0.6 -0.1 N.D. -0.1
growth on ricinoleic acid YNB Ricinoleic Acid +0.3 +0.6 -0.5 N.D. -0.1
growth on oleic acid YNB Glucose -0.0 +0.3 +0.2 N.D. +0.1
auxotrophy YNB Glucose 75mM Methionine +0.2 +0.3 -0.1 N.D. +0.3
auxotrophy YNB Glucose 75mM Arginine +0.3 +0.4 -0.1 N.D. +0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.2 +0.7 +0.4 N.D. -0.1
growth on ricinoleic acid YNB Glucose +0.1 +0.8 -0.3 N.D. +0.3
auxotrophy Dropout Complete +0.3 +1.0 -0.1 N.D. +0.0
auxotrophy YNB Glucose +0.5 +0.7 -0.0 N.D. +0.1
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