Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 12148 and 12149 are separated by 1587 nucleotides12149 and 12150 are separated by 353 nucleotides12150 and 12151 are separated by 926 nucleotides12151 and 12152 are separated by 510 nucleotides mRNA_3780: 12148 - KOG1426 FOG RCC1 domain, at 561,594 to 563,970 12148 mRNA_3781: 12149 - K12821 PRPF40, PRP40 pre-mRNA-processing factor 40, at 565,558 to 568,249 12149 mRNA_3782: 12150 - K04354 PPP2R2 serine/threonine-protein phosphatase 2A regulatory subunit B, at 568,603 to 571,218 12150 mRNA_3783: 12151 - KOG1502 Flavonol reductase/cinnamoyl-CoA reductase, at 572,145 to 573,439 12151 mRNA_3784: 12152 - KOG1188 WD40 repeat protein, at 573,950 to 575,324 12152
Group Condition mRNA_3780 mRNA_3781 mRNA_3782 mRNA_3783 mRNA_3784
12148 12149 12150 12151 12152
facs on lipid accumulation Sorted No BODIPY Gate -0.2 -0.4 N.D. +0.1 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.0 +0.0 N.D. -0.4 N.D.
facs on lipid accumulation Sorted High BODIPY Gate -0.1 -0.2 N.D. -0.0 N.D.
facs on lipid accumulation Sorted Low BODIPY Gate -0.3 -0.0 N.D. +0.1 N.D.
growth on oleic acid YNB Oleic Acid +0.1 -0.3 N.D. +0.0 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.0 +0.1 N.D. -0.2 N.D.
ypd buoyancy 40 hrs No Buoyancy Separation -0.8 -0.0 N.D. +0.7 N.D.
growth on ricinoleic acid YNB Ricinoleic Acid -0.1 +0.0 N.D. +0.1 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.1 +0.2 N.D. -0.1 N.D.
growth on ypd YPD -0.0 +0.0 N.D. +0.2 N.D.
ypd buoyancy 40 hrs Low Buoyancy +0.1 +0.1 N.D. +0.1 N.D.
ypd buoyancy 40 hrs High Buoyancy -0.0 +0.0 N.D. +0.4 N.D.
growth on ricinoleic acid YNB Glucose +0.3 +0.3 N.D. -0.2 N.D.
growth on oleic acid YNB Glucose +0.1 +0.1 N.D. +0.2 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid +0.3 +0.1 N.D. +0.2 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.1 +0.3 N.D. +0.4 N.D.
ypd buoyancy 40 hrs Median Buoyancy +0.1 +0.0 N.D. +0.4 N.D.
auxotrophy YNB Glucose +0.1 +0.5 N.D. +0.5 N.D.
auxotrophy YNB Glucose 75mM Arginine +0.1 +0.7 N.D. +0.5 N.D.
auxotrophy Dropout Complete +0.0 +0.7 N.D. +0.8 N.D.
auxotrophy YNB Glucose 75mM Methionine +0.3 +0.9 N.D. +0.5 N.D.
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