Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 12149 and 12150 are separated by 353 nucleotides12150 and 12151 are separated by 926 nucleotides12151 and 12152 are separated by 510 nucleotides12152 and 12153 are separated by 357 nucleotides mRNA_3781: 12149 - K12821 PRPF40, PRP40 pre-mRNA-processing factor 40, at 565,558 to 568,249 12149 mRNA_3782: 12150 - K04354 PPP2R2 serine/threonine-protein phosphatase 2A regulatory subunit B, at 568,603 to 571,218 12150 mRNA_3783: 12151 - KOG1502 Flavonol reductase/cinnamoyl-CoA reductase, at 572,145 to 573,439 12151 mRNA_3784: 12152 - KOG1188 WD40 repeat protein, at 573,950 to 575,324 12152 mRNA_3785: 12153 - K15178 RTF1 RNA polymerase-associated protein RTF1, at 575,682 to 577,961 12153
Group Condition mRNA_3781 mRNA_3782 mRNA_3783 mRNA_3784 mRNA_3785
12149 12150 12151 12152 12153
facs on lipid accumulation Sorted Low BODIPY Gate -0.0 N.D. +0.1 N.D. -2.3
facs on lipid accumulation Sorted No BODIPY Gate -0.4 N.D. +0.1 N.D. -1.0
growth on ricinoleic acid YNB Ricinoleic Acid +0.0 N.D. +0.1 N.D. -1.2
facs on lipid accumulation Sorted High BODIPY Gate -0.2 N.D. -0.0 N.D. -0.5
growth on ricinoleic acid YNB Glucose +0.3 N.D. -0.2 N.D. -0.7
growth on ypd YPD +0.0 N.D. +0.2 N.D. -0.7
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.0 N.D. -0.4 N.D. +0.3
growth on ricinoleic acid YNB Methylricinoleic Acid +0.1 N.D. +0.2 N.D. -0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.1 N.D. -0.2 N.D. +0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.3 N.D. +0.4 N.D. -0.5
growth on oleic acid YNB Oleic Acid -0.3 N.D. +0.0 N.D. +0.5
ypd buoyancy 40 hrs No Buoyancy Separation -0.0 N.D. +0.7 N.D. -0.5
ypd buoyancy 40 hrs High Buoyancy +0.0 N.D. +0.4 N.D. -0.1
ypd buoyancy 40 hrs Median Buoyancy +0.0 N.D. +0.4 N.D. +0.0
ypd buoyancy 40 hrs Low Buoyancy +0.1 N.D. +0.1 N.D. +0.6
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.2 N.D. -0.1 N.D. +0.7
growth on oleic acid YNB Glucose +0.1 N.D. +0.2 N.D. +0.6
auxotrophy YNB Glucose +0.5 N.D. +0.5 N.D. -0.1
auxotrophy YNB Glucose 75mM Arginine +0.7 N.D. +0.5 N.D. -0.1
auxotrophy YNB Glucose 75mM Methionine +0.9 N.D. +0.5 N.D. +0.4
auxotrophy Dropout Complete +0.7 N.D. +0.8 N.D. +0.3
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