Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 12151 and 12152 are separated by 510 nucleotides12152 and 12153 are separated by 357 nucleotides12153 and 12154 are separated by 515 nucleotides12154 and 12155 are separated by 977 nucleotides mRNA_3783: 12151 - KOG1502 Flavonol reductase/cinnamoyl-CoA reductase, at 572,145 to 573,439 12151 mRNA_3784: 12152 - KOG1188 WD40 repeat protein, at 573,950 to 575,324 12152 mRNA_3785: 12153 - K15178 RTF1 RNA polymerase-associated protein RTF1, at 575,682 to 577,961 12153 mRNA_3786: 12154 - K00006 GPD1 glycerol-3-phosphate dehydrogenase (NAD+), at 578,477 to 580,207 12154 mRNA_3787: 12155 - K01626 E2.5.1.54, aroF, aroG, aroH 3-deoxy-7-phosphoheptulonate synthase, at 581,185 to 583,154 12155
Group Condition mRNA_3783 mRNA_3784 mRNA_3785 mRNA_3786 mRNA_3787
12151 12152 12153 12154 12155
growth on ricinoleic acid YNB Ricinoleic Acid +0.1 N.D. -1.2 -2.9 -0.0
growth on ricinoleic acid YNB Methylricinoleic Acid +0.2 N.D. -0.2 -4.2 +0.3
facs on lipid accumulation Sorted High BODIPY Gate -0.0 N.D. -0.5 -2.6 +0.3
facs on lipid accumulation Sorted No BODIPY Gate +0.1 N.D. -1.0 -1.7 -0.1
ypd buoyancy 40 hrs No Buoyancy Separation +0.7 N.D. -0.5 -1.3 -0.7
growth on ricinoleic acid YNB Glucose -0.2 N.D. -0.7 -0.3 -0.0
facs on lipid accumulation Sorted Low BODIPY Gate +0.1 N.D. -2.3 +1.4 -0.2
growth on ypd YPD +0.2 N.D. -0.7 -0.1 -0.3
growth on oleic acid YNB Oleic Acid +0.0 N.D. +0.5 -1.4 +0.6
ypd buoyancy 40 hrs Low Buoyancy +0.1 N.D. +0.6 -1.0 +0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.4 N.D. +0.3 -0.3 +0.3
ypd buoyancy 40 hrs High Buoyancy +0.4 N.D. -0.1 -0.4 +0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.2 N.D. +0.2 -0.4 +0.5
auxotrophy YNB Glucose 75mM Arginine +0.5 N.D. -0.1 +0.3 -0.5
auxotrophy YNB Glucose +0.5 N.D. -0.1 +0.5 -0.3
ypd buoyancy 40 hrs Median Buoyancy +0.4 N.D. +0.0 -0.1 +0.3
auxotrophy YNB Glucose 75mM Methionine +0.5 N.D. +0.4 -0.0 -0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.1 N.D. +0.7 +0.2 -0.0
growth on oleic acid YNB Glucose +0.2 N.D. +0.6 -0.4 +0.5
auxotrophy Dropout Complete +0.8 N.D. +0.3 +0.2 -0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.4 N.D. -0.5 +1.8 +0.2
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