Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 12156 and 12157 are separated by 290 nucleotides12157 and 12158 are separated by 537 nucleotides12158 and 12159 are separated by 128 nucleotides12159 and 12160 are separated by 414 nucleotides mRNA_3788: 12156 - Hypothetical Protein, at 583,211 to 584,056 12156 mRNA_3789: 12157 - K09584 PDIA6, TXNDC7 protein disulfide-isomerase A6, at 584,347 to 585,517 12157 mRNA_3790: 12158 - K07151 STT3 dolichyl-diphosphooligosaccharide--protein glycosyltransferase, at 586,055 to 588,728 12158 mRNA_3791: 12159 - K00287 folA dihydrofolate reductase, at 588,857 to 589,593 12159 mRNA_3792: 12160 - K12811 DDX46, PRP5 ATP-dependent RNA helicase DDX46/PRP5, at 590,008 to 594,469 12160
Group Condition mRNA_3788 mRNA_3789 mRNA_3790 mRNA_3791 mRNA_3792
12156 12157 12158 12159 12160
facs on lipid accumulation Sorted Low BODIPY Gate -1.4 +0.0 N.D. N.D. +0.5
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -1.0 -0.2 N.D. N.D. +0.4
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.5 -0.2 N.D. N.D. +0.2
ypd buoyancy 40 hrs No Buoyancy Separation -0.8 -0.1 N.D. N.D. +0.6
growth on ricinoleic acid YNB Methylricinoleic Acid +0.0 +1.0 N.D. N.D. -1.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.4 +0.1 N.D. N.D. +0.3
auxotrophy YNB Glucose -0.2 +0.2 N.D. N.D. +0.1
facs on lipid accumulation Sorted High BODIPY Gate -0.2 -0.4 N.D. N.D. +0.7
growth on ricinoleic acid YNB Ricinoleic Acid -0.6 +1.5 N.D. N.D. -0.8
ypd buoyancy 40 hrs High Buoyancy -0.2 -0.0 N.D. N.D. +0.5
ypd buoyancy 40 hrs Low Buoyancy -0.1 +0.2 N.D. N.D. +0.1
auxotrophy Dropout Complete +0.2 +0.2 N.D. N.D. +0.1
growth on ricinoleic acid YNB Glucose -0.4 +0.4 N.D. N.D. +0.6
growth on oleic acid YNB Oleic Acid +0.2 +0.3 N.D. N.D. +0.2
auxotrophy YNB Glucose 75mM Methionine -0.1 +0.3 N.D. N.D. +0.4
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.1 +0.1 N.D. N.D. +0.6
facs on lipid accumulation Sorted No BODIPY Gate +0.1 +0.1 N.D. N.D. +0.6
auxotrophy YNB Glucose 75mM Arginine +0.2 +0.2 N.D. N.D. +0.5
ypd buoyancy 40 hrs Median Buoyancy +0.2 +0.5 N.D. N.D. +0.2
growth on oleic acid YNB Glucose +0.2 +0.2 N.D. N.D. +0.6
growth on ypd YPD +1.0 +0.3 N.D. N.D. +0.6
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