Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 12157 and 12158 are separated by 537 nucleotides12158 and 12159 are separated by 128 nucleotides12159 and 12160 are separated by 414 nucleotides12160 and 12161 are separated by 343 nucleotides mRNA_3789: 12157 - K09584 PDIA6, TXNDC7 protein disulfide-isomerase A6, at 584,347 to 585,517 12157 mRNA_3790: 12158 - K07151 STT3 dolichyl-diphosphooligosaccharide--protein glycosyltransferase, at 586,055 to 588,728 12158 mRNA_3791: 12159 - K00287 folA dihydrofolate reductase, at 588,857 to 589,593 12159 mRNA_3792: 12160 - K12811 DDX46, PRP5 ATP-dependent RNA helicase DDX46/PRP5, at 590,008 to 594,469 12160 mRNA_3793: 12161 - K15528 FAAH fatty acid amide hydrolase, at 594,813 to 596,952 12161
Group Condition mRNA_3789 mRNA_3790 mRNA_3791 mRNA_3792 mRNA_3793
12157 12158 12159 12160 12161
growth on ricinoleic acid YNB Methylricinoleic Acid +1.0 N.D. N.D. -1.1 -0.4
facs on lipid accumulation Sorted High BODIPY Gate -0.4 N.D. N.D. +0.7 -0.6
auxotrophy YNB Glucose +0.2 N.D. N.D. +0.1 -0.3
ypd buoyancy 40 hrs Low Buoyancy +0.2 N.D. N.D. +0.1 -0.2
growth on oleic acid YNB Oleic Acid +0.3 N.D. N.D. +0.2 -0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.2 N.D. N.D. +0.2 +0.2
facs on lipid accumulation Sorted No BODIPY Gate +0.1 N.D. N.D. +0.6 -0.5
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.2 N.D. N.D. +0.4 +0.1
auxotrophy Dropout Complete +0.2 N.D. N.D. +0.1 +0.0
ypd buoyancy 40 hrs No Buoyancy Separation -0.1 N.D. N.D. +0.6 -0.1
ypd buoyancy 40 hrs High Buoyancy -0.0 N.D. N.D. +0.5 -0.0
auxotrophy YNB Glucose 75mM Methionine +0.3 N.D. N.D. +0.4 -0.3
facs on lipid accumulation Sorted Low BODIPY Gate +0.0 N.D. N.D. +0.5 -0.1
growth on ricinoleic acid YNB Glucose +0.4 N.D. N.D. +0.6 -0.4
auxotrophy YNB Glucose 75mM Arginine +0.2 N.D. N.D. +0.5 -0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.1 N.D. N.D. +0.3 +0.2
ypd buoyancy 40 hrs Median Buoyancy +0.5 N.D. N.D. +0.2 -0.0
growth on oleic acid YNB Glucose +0.2 N.D. N.D. +0.6 -0.0
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.1 N.D. N.D. +0.6 +0.1
growth on ricinoleic acid YNB Ricinoleic Acid +1.5 N.D. N.D. -0.8 +0.2
growth on ypd YPD +0.3 N.D. N.D. +0.6 +0.3
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