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Fitness for 5 genes in
Rhodosporidium toruloides IFO0880
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Top 21 experiments (either direction), sorted by average fitness
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500 nt
12157 and 12158 are separated by 537 nucleotides
12158 and 12159 are separated by 128 nucleotides
12159 and 12160 are separated by 414 nucleotides
12160 and 12161 are separated by 343 nucleotides
mRNA_3789: 12157 - K09584 PDIA6, TXNDC7 protein disulfide-isomerase A6, at 584,347 to 585,517
12157
mRNA_3790: 12158 - K07151 STT3 dolichyl-diphosphooligosaccharide--protein glycosyltransferase, at 586,055 to 588,728
12158
mRNA_3791: 12159 - K00287 folA dihydrofolate reductase, at 588,857 to 589,593
12159
mRNA_3792: 12160 - K12811 DDX46, PRP5 ATP-dependent RNA helicase DDX46/PRP5, at 590,008 to 594,469
12160
mRNA_3793: 12161 - K15528 FAAH fatty acid amide hydrolase, at 594,813 to 596,952
12161
Group
Condition
mRNA
_3789
mRNA
_3790
mRNA
_3791
mRNA
_3792
mRNA
_3793
12157
12158
12159
12160
12161
growth on ricinoleic acid
YNB Methylricinoleic Acid
+1.0
N.D.
N.D.
-1.1
-0.4
facs on lipid accumulation
Sorted High BODIPY Gate
-0.4
N.D.
N.D.
+0.7
-0.6
auxotrophy
YNB Glucose
+0.2
N.D.
N.D.
+0.1
-0.3
ypd buoyancy
40 hrs Low Buoyancy
+0.2
N.D.
N.D.
+0.1
-0.2
growth on oleic acid
YNB Oleic Acid
+0.3
N.D.
N.D.
+0.2
-0.2
buoyancy on lipid accumulation
40 hrs Median Buoyancy
-0.2
N.D.
N.D.
+0.2
+0.2
facs on lipid accumulation
Sorted No BODIPY Gate
+0.1
N.D.
N.D.
+0.6
-0.5
buoyancy on lipid accumulation
40 hrs No Buoyancy Separation
-0.2
N.D.
N.D.
+0.4
+0.1
auxotrophy
Dropout Complete
+0.2
N.D.
N.D.
+0.1
+0.0
ypd buoyancy
40 hrs No Buoyancy Separation
-0.1
N.D.
N.D.
+0.6
-0.1
ypd buoyancy
40 hrs High Buoyancy
-0.0
N.D.
N.D.
+0.5
-0.0
auxotrophy
YNB Glucose 75mM Methionine
+0.3
N.D.
N.D.
+0.4
-0.3
facs on lipid accumulation
Sorted Low BODIPY Gate
+0.0
N.D.
N.D.
+0.5
-0.1
growth on ricinoleic acid
YNB Glucose
+0.4
N.D.
N.D.
+0.6
-0.4
auxotrophy
YNB Glucose 75mM Arginine
+0.2
N.D.
N.D.
+0.5
-0.1
buoyancy on lipid accumulation
40 hrs Low Buoyancy
+0.1
N.D.
N.D.
+0.3
+0.2
ypd buoyancy
40 hrs Median Buoyancy
+0.5
N.D.
N.D.
+0.2
-0.0
growth on oleic acid
YNB Glucose
+0.2
N.D.
N.D.
+0.6
-0.0
buoyancy on lipid accumulation
40 hrs High Buoyancy
+0.1
N.D.
N.D.
+0.6
+0.1
growth on ricinoleic acid
YNB Ricinoleic Acid
+1.5
N.D.
N.D.
-0.8
+0.2
growth on ypd
YPD
+0.3
N.D.
N.D.
+0.6
+0.3
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