Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 12170 and 12171 are separated by 965 nucleotides12171 and 12172 are separated by 320 nucleotides12172 and 12173 are separated by 113 nucleotides12173 and 12174 are separated by 140 nucleotides mRNA_3802: 12170 - K01939 purA, ADSS adenylosuccinate synthase, at 611,047 to 612,951 12170 mRNA_3803: 12171 - Hypothetical Protein, at 613,917 to 616,021 12171 mRNA_3804: 12172 - Hypothetical Protein, at 616,342 to 617,222 12172 mRNA_3805: 12173 - Hypothetical Protein, at 617,336 to 618,142 12173 mRNA_3806: 12174 - K02365 ESP1 separase, at 618,283 to 626,082 12174
Group Condition mRNA_3802 mRNA_3803 mRNA_3804 mRNA_3805 mRNA_3806
12170 12171 12172 12173 12174
facs on lipid accumulation Sorted Low BODIPY Gate N.D. -0.5 -0.2 N.D. N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. -0.2 -0.1 N.D. N.D.
ypd buoyancy 40 hrs High Buoyancy N.D. +0.1 -0.3 N.D. N.D.
growth on oleic acid YNB Glucose N.D. -0.0 -0.2 N.D. N.D.
auxotrophy Dropout Complete N.D. -0.1 -0.1 N.D. N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. -0.1 -0.1 N.D. N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. -0.1 -0.0 N.D. N.D.
auxotrophy YNB Glucose N.D. +0.1 -0.3 N.D. N.D.
ypd buoyancy 40 hrs Median Buoyancy N.D. -0.2 +0.1 N.D. N.D.
ypd buoyancy 40 hrs No Buoyancy Separation N.D. -0.3 +0.2 N.D. N.D.
ypd buoyancy 40 hrs Low Buoyancy N.D. +0.1 -0.1 N.D. N.D.
growth on oleic acid YNB Oleic Acid N.D. +0.2 -0.2 N.D. N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. -0.1 +0.1 N.D. N.D.
auxotrophy YNB Glucose 75mM Arginine N.D. +0.1 -0.1 N.D. N.D.
facs on lipid accumulation Sorted No BODIPY Gate N.D. -0.1 +0.2 N.D. N.D.
growth on ricinoleic acid YNB Glucose N.D. +0.4 -0.3 N.D. N.D.
facs on lipid accumulation Sorted High BODIPY Gate N.D. -0.0 +0.3 N.D. N.D.
auxotrophy YNB Glucose 75mM Methionine N.D. +0.3 +0.0 N.D. N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. +0.6 -0.1 N.D. N.D.
growth on ricinoleic acid YNB Ricinoleic Acid N.D. +0.8 -0.2 N.D. N.D.
growth on ypd YPD N.D. +0.7 +0.5 N.D. N.D.
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