Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 12213 and 12214 are separated by 988 nucleotides12214 and 12215 are separated by 1804 nucleotides12215 and 12216 are separated by 513 nucleotides12216 and 12217 are separated by 360 nucleotides mRNA_3845: 12213 - HMMPfam-Putative methyltransferase-PF10294,SUPERFAMILY--SSF53335, at 726,085 to 727,619 12213 mRNA_3846: 12214 - HMMPfam-HORMA domain-PF02301,HMMPfam-Zinc-finger double-stranded RNA-binding-PF12171,ProSitePatterns-Zinc finger C2H2 type domain signature.-PS00028,ProSiteProfiles-Zinc finger C2H2 type domain profile.-PS50157,ProSiteProfiles-HORMA domain profile.-PS50815,SMART-zinc finger-SM00355,SUPERFAMILY--SSF56019,SUPERFAMILY--SSF57667, at 728,608 to 730,842 12214 mRNA_3847: 12215 - Hypothetical Protein, at 732,647 to 733,359 12215 mRNA_3848: 12216 - KOG1817 Ribonuclease, at 733,873 to 734,892 12216 mRNA_3849: 12217 - Hypothetical Protein, at 735,253 to 736,177 12217
Group Condition mRNA_3845 mRNA_3846 mRNA_3847 mRNA_3848 mRNA_3849
12213 12214 12215 12216 12217
growth on ypd YPD -0.3 -0.0 N.D. -0.8 -0.1
facs on lipid accumulation Sorted Low BODIPY Gate -0.3 +0.0 N.D. -0.4 +0.1
growth on ricinoleic acid YNB Methylricinoleic Acid -0.1 +0.1 N.D. +0.0 -0.1
ypd buoyancy 40 hrs Low Buoyancy +0.0 -0.1 N.D. +0.3 -0.0
facs on lipid accumulation Sorted No BODIPY Gate +0.2 -0.0 N.D. -0.1 +0.1
ypd buoyancy 40 hrs High Buoyancy -0.3 +0.0 N.D. +0.7 -0.2
auxotrophy YNB Glucose 75mM Methionine +0.1 +0.1 N.D. +0.3 -0.1
growth on ricinoleic acid YNB Ricinoleic Acid +0.2 -0.0 N.D. +0.3 -0.1
facs on lipid accumulation Sorted High BODIPY Gate -0.2 -0.1 N.D. +0.4 +0.4
ypd buoyancy 40 hrs No Buoyancy Separation +0.3 +0.0 N.D. +0.0 +0.2
auxotrophy Dropout Complete +0.4 -0.1 N.D. +0.3 -0.1
ypd buoyancy 40 hrs Median Buoyancy +0.2 -0.1 N.D. +0.4 +0.0
growth on oleic acid YNB Oleic Acid +0.3 +0.0 N.D. +0.3 +0.0
growth on ricinoleic acid YNB Glucose +0.0 +0.1 N.D. +0.7 -0.1
auxotrophy YNB Glucose +0.6 +0.1 N.D. +0.3 -0.2
auxotrophy YNB Glucose 75mM Arginine +0.5 -0.1 N.D. +0.6 -0.2
growth on oleic acid YNB Glucose +0.2 +0.2 N.D. +0.4 +0.0
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.2 -0.1 N.D. +1.5 -0.0
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.1 -0.4 N.D. +1.6 -0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.0 -0.1 N.D. +1.4 -0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.2 +0.2 N.D. +1.6 -0.0
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