Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 12214 and 12215 are separated by 1804 nucleotides12215 and 12216 are separated by 513 nucleotides12216 and 12217 are separated by 360 nucleotides12217 and 12218 are separated by 2497 nucleotides mRNA_3846: 12214 - HMMPfam-HORMA domain-PF02301,HMMPfam-Zinc-finger double-stranded RNA-binding-PF12171,ProSitePatterns-Zinc finger C2H2 type domain signature.-PS00028,ProSiteProfiles-Zinc finger C2H2 type domain profile.-PS50157,ProSiteProfiles-HORMA domain profile.-PS50815,SMART-zinc finger-SM00355,SUPERFAMILY--SSF56019,SUPERFAMILY--SSF57667, at 728,608 to 730,842 12214 mRNA_3847: 12215 - Hypothetical Protein, at 732,647 to 733,359 12215 mRNA_3848: 12216 - KOG1817 Ribonuclease, at 733,873 to 734,892 12216 mRNA_3849: 12217 - Hypothetical Protein, at 735,253 to 736,177 12217 mRNA_3850: 12218 - BLAST Protein kinase-like domain protein [Cordyceps confrag..., at 738,675 to 741,507 12218
Group Condition mRNA_3846 mRNA_3847 mRNA_3848 mRNA_3849 mRNA_3850
12214 12215 12216 12217 12218
growth on ypd YPD -0.0 N.D. -0.8 -0.1 +0.1
growth on ricinoleic acid YNB Methylricinoleic Acid +0.1 N.D. +0.0 -0.1 -0.2
facs on lipid accumulation Sorted Low BODIPY Gate +0.0 N.D. -0.4 +0.1 -0.0
facs on lipid accumulation Sorted No BODIPY Gate -0.0 N.D. -0.1 +0.1 -0.2
ypd buoyancy 40 hrs Low Buoyancy -0.1 N.D. +0.3 -0.0 -0.2
auxotrophy Dropout Complete -0.1 N.D. +0.3 -0.1 -0.1
auxotrophy YNB Glucose +0.1 N.D. +0.3 -0.2 -0.2
auxotrophy YNB Glucose 75mM Methionine +0.1 N.D. +0.3 -0.1 +0.1
ypd buoyancy 40 hrs Median Buoyancy -0.1 N.D. +0.4 +0.0 +0.1
ypd buoyancy 40 hrs No Buoyancy Separation +0.0 N.D. +0.0 +0.2 +0.2
ypd buoyancy 40 hrs High Buoyancy +0.0 N.D. +0.7 -0.2 -0.0
auxotrophy YNB Glucose 75mM Arginine -0.1 N.D. +0.6 -0.2 +0.2
growth on oleic acid YNB Oleic Acid +0.0 N.D. +0.3 +0.0 +0.3
facs on lipid accumulation Sorted High BODIPY Gate -0.1 N.D. +0.4 +0.4 +0.2
growth on oleic acid YNB Glucose +0.2 N.D. +0.4 +0.0 +0.2
growth on ricinoleic acid YNB Ricinoleic Acid -0.0 N.D. +0.3 -0.1 +0.7
growth on ricinoleic acid YNB Glucose +0.1 N.D. +0.7 -0.1 +0.4
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.4 N.D. +1.6 -0.1 +0.4
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.1 N.D. +1.4 -0.1 +0.5
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.1 N.D. +1.5 -0.0 +0.6
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.2 N.D. +1.6 -0.0 +0.6
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