Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 12216 and 12217 are separated by 360 nucleotides12217 and 12218 are separated by 2497 nucleotides12218 and 12219 are separated by 547 nucleotides12219 and 12220 are separated by 273 nucleotides mRNA_3848: 12216 - KOG1817 Ribonuclease, at 733,873 to 734,892 12216 mRNA_3849: 12217 - Hypothetical Protein, at 735,253 to 736,177 12217 mRNA_3850: 12218 - BLAST Protein kinase-like domain protein [Cordyceps confrag..., at 738,675 to 741,507 12218 mRNA_3851: 12219 - HMMPfam-Major Facilitator Superfamily-PF07690,ProSiteProfiles-Major facilitator superfamily (MFS) profile.-PS50850,SUPERFAMILY--SSF103473, at 742,055 to 744,016 12219 mRNA_3852: 12220 - HMMPfam-Glutathione S-transferase, C-terminal domain-PF00043,HMMPfam-Glutathione S-transferase, N-terminal domain-PF13417,ProSiteProfiles-Soluble glutathione S-transferase N-terminal domain profile.-PS50404,ProSiteProfiles-Soluble glutathione S-transferase C-terminal domain profile.-PS50405,SUPERFAMILY--SSF47616,SUPERFAMILY--SSF52833, at 744,290 to 745,525 12220
Group Condition mRNA_3848 mRNA_3849 mRNA_3850 mRNA_3851 mRNA_3852
12216 12217 12218 12219 12220
growth on ypd YPD -0.8 -0.1 +0.1 -0.2 -0.4
growth on ricinoleic acid YNB Methylricinoleic Acid +0.0 -0.1 -0.2 +0.2 -0.6
ypd buoyancy 40 hrs No Buoyancy Separation +0.0 +0.2 +0.2 -0.4 -0.4
facs on lipid accumulation Sorted No BODIPY Gate -0.1 +0.1 -0.2 +0.0 -0.0
growth on oleic acid YNB Glucose +0.4 +0.0 +0.2 -0.5 -0.3
facs on lipid accumulation Sorted Low BODIPY Gate -0.4 +0.1 -0.0 +0.0 +0.3
ypd buoyancy 40 hrs Low Buoyancy +0.3 -0.0 -0.2 +0.0 -0.0
auxotrophy YNB Glucose 75mM Methionine +0.3 -0.1 +0.1 +0.1 -0.2
ypd buoyancy 40 hrs Median Buoyancy +0.4 +0.0 +0.1 -0.0 -0.2
growth on oleic acid YNB Oleic Acid +0.3 +0.0 +0.3 -0.1 -0.2
auxotrophy Dropout Complete +0.3 -0.1 -0.1 +0.5 -0.1
ypd buoyancy 40 hrs High Buoyancy +0.7 -0.2 -0.0 +0.2 -0.1
auxotrophy YNB Glucose +0.3 -0.2 -0.2 +0.6 +0.0
auxotrophy YNB Glucose 75mM Arginine +0.6 -0.2 +0.2 +0.3 -0.0
facs on lipid accumulation Sorted High BODIPY Gate +0.4 +0.4 +0.2 +0.5 -0.5
growth on ricinoleic acid YNB Glucose +0.7 -0.1 +0.4 +0.1 -0.1
growth on ricinoleic acid YNB Ricinoleic Acid +0.3 -0.1 +0.7 +0.1 +0.0
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +1.6 -0.1 +0.4 -0.5 +0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy +1.4 -0.1 +0.5 +0.4 -0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy +1.6 -0.0 +0.6 +0.2 +0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy +1.5 -0.0 +0.6 +0.6 +0.0
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