Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 12232 and 12233 are separated by 1356 nucleotides12233 and 12234 are separated by 1688 nucleotides12234 and 12235 are separated by 1159 nucleotides12235 and 12236 are separated by 254 nucleotides mRNA_3864: 12232 - K08737 MSH6 DNA mismatch repair protein MSH6, at 771,424 to 775,679 12232 mRNA_3865: 12233 - HMMPfam-DASH complex subunit Spc34-PF08657, at 777,036 to 778,951 12233 mRNA_3866: 12234 - Hypothetical Protein, at 780,640 to 781,665 12234 mRNA_3867: 12235 - KOG1812 Predicted E3 ubiquitin ligase, at 782,825 to 783,717 12235 mRNA_3868: 12236 - KOG0603 Ribosomal protein S6 kinase, at 783,972 to 787,068 12236
Group Condition mRNA_3864 mRNA_3865 mRNA_3866 mRNA_3867 mRNA_3868
12232 12233 12234 12235 12236
facs on lipid accumulation Sorted Low BODIPY Gate +0.1 -1.8 N.D. -0.5 +0.5
facs on lipid accumulation Sorted High BODIPY Gate +0.1 -1.4 N.D. -0.2 -0.0
auxotrophy Dropout Complete +0.0 -1.5 N.D. +0.5 -0.0
growth on ricinoleic acid YNB Glucose +0.1 -0.9 N.D. -0.2 -0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.1 -1.1 N.D. +0.0 +0.1
growth on ricinoleic acid YNB Methylricinoleic Acid +0.1 -0.8 N.D. -0.1 +0.1
auxotrophy YNB Glucose 75mM Methionine +0.1 -0.8 N.D. +0.1 -0.2
auxotrophy YNB Glucose +0.0 -0.7 N.D. +0.1 -0.0
growth on oleic acid YNB Glucose -0.0 +0.0 N.D. -0.6 +0.1
facs on lipid accumulation Sorted No BODIPY Gate -0.0 -1.1 N.D. +0.3 +0.4
auxotrophy YNB Glucose 75mM Arginine +0.1 -0.8 N.D. +0.3 +0.1
ypd buoyancy 40 hrs No Buoyancy Separation +0.1 +0.1 N.D. +0.0 -0.3
growth on ricinoleic acid YNB Ricinoleic Acid +0.3 -0.8 N.D. +0.2 +0.3
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.1 +0.1 N.D. -0.1 -0.2
growth on oleic acid YNB Oleic Acid +0.1 +0.6 N.D. -0.8 +0.3
growth on ypd YPD -0.1 +0.7 N.D. -0.4 -0.0
ypd buoyancy 40 hrs High Buoyancy +0.2 -0.1 N.D. +0.4 -0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.1 -0.0 N.D. +0.1 +0.2
ypd buoyancy 40 hrs Low Buoyancy +0.1 -0.3 N.D. +0.5 +0.2
ypd buoyancy 40 hrs Median Buoyancy +0.1 +0.2 N.D. +0.4 -0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.2 +0.6 N.D. +0.1 +0.1
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