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Fitness for 5 genes in
Rhodosporidium toruloides IFO0880
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Top 21 experiments (either direction), sorted by average fitness
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500 nt
12256 and 12257 are separated by 210 nucleotides
12257 and 12258 are separated by 412 nucleotides
12258 and 12259 are separated by 215 nucleotides
12259 and 12260 are separated by 1456 nucleotides
mRNA_3888: 12256 - HMMPfam-GDSL-like Lipase/Acylhydrolase family-PF13472,SUPERFAMILY--SSF52266, at 829,400 to 831,173
12256
mRNA_3889: 12257 - K00587 ICMT, STE14 protein-S-isoprenylcysteine O-methyltransferase, at 831,384 to 832,562
12257
mRNA_3890: 12258 - HMMPfam-Pal1 cell morphology protein-PF08316, at 832,975 to 835,014
12258
mRNA_3891: 12259 - K07889 RAB5C Ras-related protein Rab-5C, at 835,230 to 836,530
12259
mRNA_3892: 12260 - Hypothetical Protein, at 837,987 to 839,368
12260
Group
Condition
mRNA
_3888
mRNA
_3889
mRNA
_3890
mRNA
_3891
mRNA
_3892
12256
12257
12258
12259
12260
growth on ricinoleic acid
YNB Ricinoleic Acid
-0.1
-1.4
+0.8
-2.1
+0.4
growth on ypd
YPD
+0.1
-1.1
-0.0
-0.7
-0.2
facs on lipid accumulation
Sorted High BODIPY Gate
+0.3
-1.1
+0.0
-1.0
-0.0
growth on ricinoleic acid
YNB Methylricinoleic Acid
-0.1
-1.0
+1.1
-1.3
+0.1
buoyancy on lipid accumulation
40 hrs No Buoyancy Separation
-0.2
-0.0
-0.3
-0.5
-0.1
facs on lipid accumulation
Sorted Low BODIPY Gate
+0.1
-0.8
+0.0
-0.9
+0.5
growth on oleic acid
YNB Glucose
-0.3
-0.0
+0.5
-1.3
+0.2
growth on ricinoleic acid
YNB Glucose
-0.1
-1.0
+0.7
-0.5
+0.1
facs on lipid accumulation
Sorted No BODIPY Gate
+0.1
-0.4
-0.1
-0.7
+0.5
ypd buoyancy
40 hrs No Buoyancy Separation
-0.3
-0.6
-0.5
+1.5
-0.5
buoyancy on lipid accumulation
40 hrs Median Buoyancy
+0.0
-0.0
-0.2
-0.4
+0.1
buoyancy on lipid accumulation
40 hrs Low Buoyancy
+0.0
+0.0
+0.1
-0.3
-0.0
auxotrophy
YNB Glucose 75mM Arginine
-0.3
+0.0
+0.9
-0.7
+0.1
auxotrophy
YNB Glucose
+0.1
-0.4
+1.0
-0.5
+0.2
growth on oleic acid
YNB Oleic Acid
-0.2
+0.4
+0.7
-0.5
+0.2
auxotrophy
Dropout Complete
+0.1
-0.1
+0.9
-0.3
+0.2
buoyancy on lipid accumulation
40 hrs High Buoyancy
+0.4
+0.4
+0.3
-0.3
+0.1
ypd buoyancy
40 hrs Median Buoyancy
+0.0
-0.0
-0.0
+0.9
+0.3
ypd buoyancy
40 hrs Low Buoyancy
-0.2
+0.1
+0.2
+0.8
+0.2
ypd buoyancy
40 hrs High Buoyancy
+0.4
+0.4
-0.3
+0.7
+0.3
auxotrophy
YNB Glucose 75mM Methionine
+0.2
-0.2
+1.0
+0.5
+0.1
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