Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 12269 and 12270 are separated by 663 nucleotides12270 and 12271 are separated by 296 nucleotides12271 and 12272 are separated by 49 nucleotides12272 and 12273 are separated by 619 nucleotides mRNA_3901: 12269 - K00863 DAK, TKFC triose/dihydroxyacetone kinase / FAD-AMP lyase (cyclizing), at 852,096 to 854,470 12269 mRNA_3902: 12270 - K12471 EPN epsin, at 855,134 to 857,273 12270 mRNA_3903: 12271 - K01273 DPEP membrane dipeptidase, at 857,570 to 859,111 12271 mRNA_3904: 12272 - KOG1176 Acyl-CoA synthetase, at 859,161 to 861,426 12272 mRNA_3905: 12273 - KOG0118 FOG RRM domain, at 862,046 to 863,996 12273
Group Condition mRNA_3901 mRNA_3902 mRNA_3903 mRNA_3904 mRNA_3905
12269 12270 12271 12272 12273
facs on lipid accumulation Sorted Low BODIPY Gate +0.1 N.D. +0.3 +0.0 -1.9
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.1 N.D. -0.2 +0.0 -1.4
growth on oleic acid YNB Glucose -0.6 N.D. -0.1 -0.2 -0.2
growth on ricinoleic acid YNB Glucose -0.1 N.D. -0.3 -0.3 -0.1
auxotrophy YNB Glucose 75mM Methionine +0.1 N.D. -0.2 -0.2 -0.4
auxotrophy YNB Glucose +0.2 N.D. -0.4 -0.1 -0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.0 N.D. -0.5 +0.0 +0.0
facs on lipid accumulation Sorted No BODIPY Gate +0.3 N.D. -0.2 +0.2 -0.7
growth on oleic acid YNB Oleic Acid -0.2 N.D. -0.2 -0.3 +0.3
auxotrophy Dropout Complete +0.1 N.D. -0.2 -0.1 -0.1
auxotrophy YNB Glucose 75mM Arginine +0.2 N.D. -0.3 -0.0 -0.1
growth on ricinoleic acid YNB Methylricinoleic Acid -0.1 N.D. -0.2 -0.5 +0.5
growth on ricinoleic acid YNB Ricinoleic Acid -0.3 N.D. -0.3 -0.4 +0.8
growth on ypd YPD +0.1 N.D. +0.0 +0.1 -0.3
ypd buoyancy 40 hrs Median Buoyancy +0.2 N.D. +0.2 +0.0 -0.6
facs on lipid accumulation Sorted High BODIPY Gate +0.1 N.D. -0.2 +0.3 -0.2
ypd buoyancy 40 hrs High Buoyancy +0.0 N.D. +0.1 +0.1 -0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.1 N.D. -0.2 -0.0 +0.2
ypd buoyancy 40 hrs Low Buoyancy +0.0 N.D. +0.2 -0.1 +0.1
ypd buoyancy 40 hrs No Buoyancy Separation +0.3 N.D. +0.4 +0.3 -0.7
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.1 N.D. -0.2 +0.1 +1.1
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