Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 12270 and 12271 are separated by 296 nucleotides12271 and 12272 are separated by 49 nucleotides12272 and 12273 are separated by 619 nucleotides12273 and 12274 are separated by 1749 nucleotides mRNA_3902: 12270 - K12471 EPN epsin, at 855,134 to 857,273 12270 mRNA_3903: 12271 - K01273 DPEP membrane dipeptidase, at 857,570 to 859,111 12271 mRNA_3904: 12272 - KOG1176 Acyl-CoA synthetase, at 859,161 to 861,426 12272 mRNA_3905: 12273 - KOG0118 FOG RRM domain, at 862,046 to 863,996 12273 mRNA_3906: 12274 - K03495 gidA, mnmG, MTO1 tRNA uridine 5-carboxymethylaminomethyl modification enzyme, at 865,746 to 868,815 12274
Group Condition mRNA_3902 mRNA_3903 mRNA_3904 mRNA_3905 mRNA_3906
12270 12271 12272 12273 12274
facs on lipid accumulation Sorted Low BODIPY Gate N.D. +0.3 +0.0 -1.9 +0.3
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. -0.2 +0.0 -1.4 +0.3
growth on ricinoleic acid YNB Glucose N.D. -0.3 -0.3 -0.1 -0.3
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. -0.2 -0.5 +0.5 -0.5
auxotrophy YNB Glucose N.D. -0.4 -0.1 -0.2 +0.1
auxotrophy Dropout Complete N.D. -0.2 -0.1 -0.1 -0.1
growth on oleic acid YNB Oleic Acid N.D. -0.2 -0.3 +0.3 -0.3
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. -0.5 +0.0 +0.0 -0.0
auxotrophy YNB Glucose 75mM Arginine N.D. -0.3 -0.0 -0.1 +0.1
auxotrophy YNB Glucose 75mM Methionine N.D. -0.2 -0.2 -0.4 +0.3
growth on oleic acid YNB Glucose N.D. -0.1 -0.2 -0.2 +0.1
facs on lipid accumulation Sorted No BODIPY Gate N.D. -0.2 +0.2 -0.7 +0.4
facs on lipid accumulation Sorted High BODIPY Gate N.D. -0.2 +0.3 -0.2 -0.2
growth on ypd YPD N.D. +0.0 +0.1 -0.3 -0.0
growth on ricinoleic acid YNB Ricinoleic Acid N.D. -0.3 -0.4 +0.8 -0.4
ypd buoyancy 40 hrs Median Buoyancy N.D. +0.2 +0.0 -0.6 +0.2
ypd buoyancy 40 hrs High Buoyancy N.D. +0.1 +0.1 -0.1 -0.1
ypd buoyancy 40 hrs Low Buoyancy N.D. +0.2 -0.1 +0.1 -0.1
ypd buoyancy 40 hrs No Buoyancy Separation N.D. +0.4 +0.3 -0.7 +0.5
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. -0.2 -0.0 +0.2 +0.5
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. -0.2 +0.1 +1.1 +0.3
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